- Clone
- 5F6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Alpha4, Immunoglobulin-binding protein 1, CD79a-binding protein 1, phosphoprotein, component, 52 kD, lymphocyte signal transduction molecule alpha 4, ALPHA-4, IBP1, renal carcinoma antigen NY-REN-16
- Previously
-
Covance Catalog# MMS-5088
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
828601 | 100 µL | 220€ |
IGBP1 (also known as Alpha4) , binds to PP2A C in the absense of A and a B subunit in a catalytically inactive state to prevent C proteosomal degradation. If alpha4 levels are low, PP2A levels can decrease, leading to increased tau protein phosphorylation and aggregation. Protein phosphatase 2A is a heterotrimeric protein serine/threonine phosphatase consisting of a 36-kD catalytic C subunit (alpha and beta isoforms), a 65-kD structural A subunit (alpha and beta isoforms), and a variable regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. Ubiquitously expressed, PP2A is involved in a broad range of cellular processes, including membrane receptor desensitization, signal transduction, intermediary metabolism, transcriptional regulation and control of DNA replication, and mitosis. The PP2A phosphatase family accounts for the majority of Ser/Thr phosphatase activity in the brain and specifically regulates tau phosphorylation, both directly as tau phosphatase and indirectly by regulating the activities of several tau kinases which include CaM Kinase II, PKA, MAP kinase kinase (MEK 1/2), extracellular regulated kinase (ERK 1/2), GSK-3β, and P70S6 kinase, thus regulating tau microtubule binding in the brain.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against an N-terminal 6-HIS tagged fusion protein corresponding to full-length mouse Alpha4 protein.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
ICC, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
-
- Kong M, et al. 2009. Mol Cell. 36:51. (IP, ICC, WB)
- RRID
-
AB_2564922 (BioLegend Cat. No. 828601)
Antigen Details
- Structure
- Expected MW: 40 kD
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Phospho-Proteins
- Gene ID
- 8518 View all products for this Gene ID
- UniProt
- View information about IGBP-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All IGBP-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-IGBP-1 | 5F6 | WB,ICC,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-IGBP-1
Western blot of purified anti-IGBP-1 antibody (clone 5F6). ...
Follow Us