- Clone
- W22166E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- AA48924; Il1rak; Interleukin 1 receptor associated kinase 1; IRAK; IRAK-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
632051 | 25 µg | 136€ | ||||
632052 | 100 µg | 352€ |
The interleukin-1 receptor-associated kinase (IRAK) family of proteins are made up of serine/threonine-specific kinases which play a critical role in the response to pathogens through the induction of acute inflammation and subsequent adaptive immune responses. The mammalian IRAK molecular family consists of four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). Two are active kinases, IRAK1 and IRAK4, and two are inactive kinases, IRAK2 and IRAKM. However, all are involved in the regulation of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1R1, IL-1RAcP, MyD88, and IRAKs. Currently, there are three differentially spliced variants of IRAK1: IRAK1, IRAK1b, and IRAK1c. Auto-phosphorylation plays a role in IRAK-1 activation and mediates proteasome-mediated degradation leading to IRAK1 protein loss. Meanwhile, IRAK1b lacks kinase activity and is resistant to proteasome-mediated degradation. Additionally, IRAK1c has a truncated sequence and is therefore mutated at the C-terminus of its kinase domain and acts as a negative regulator of the TLR and IL-1R signaling pathways.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human IRAK1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For immunocytochemistry (ICC), we recommend using 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation.
This product is not recommended for use in immunohistochemistry (IHC). - Additional Product Notes
-
This antibody has been tested in knockout/knockdown models for Western Blotting (WB).
- RRID
-
AB_3097576 (BioLegend Cat. No. 632051)
AB_3097576 (BioLegend Cat. No. 632052)
Antigen Details
- Structure
- IRAK1 protein is a 712 amino acid protein with a predicted molecular weight of 77 kD
- Distribution
-
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
- Function
- Serine/threonine protein kinase
- Interaction
- TRAF6, PELI1, IRAK4, MYD88, TOLLIP, IL1RL1, IKBKG/NEMO, IRAK4, PELI3, INAVA and NFATC4
- Cell Type
- Macrophages, Monocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Dinarello CA. 1996. Blood. 87:2095-147.
- Takaesu G, et al. 2001. Mol Cell Biol. 21: 2475-84.
- Janssens S & Beyaert R. 2003. Mol Cell. 11:293-302.
- Jain A, et al. 2014. Front Immunol. 5:553.
- Gottipati S, et al. 2008. Cell Signal. 20:269-76.
- Gene ID
- 3654 View all products for this Gene ID
- UniProt
- View information about IRAK1 on UniProt.org
Related FAQs
Other Formats
View All IRAK1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-IRAK1 | W22166E | WB,ICC |
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