- Clone
- 5A1-C5-F11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mitogen-Activated Protein Kinase 14 (MAPK14), cytokine suppressive anti-inflammatory Drug Binding Protein, PRKM14, PRKM15, p38, SAPK2A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
602651 | 25 µg | 95€ | ||||
602652 | 100 µg | 235€ |
MAPK14 is a member of the p38 MAPK family of serine/threonine-specific kinases. MAPK14 plays a critical role in signal transduction, and upon activation, modulates the activity of diverse signaling families, such as the TNF, TGF, and EGFR pathways, and regulates diverse behaviors including inflammation, stress response, cell cycle, apoptosis, transcription and translation. MAPK14 is important for organogenesis, participating in the development of the heart and blood vessels.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human MAPK14 protein fragments
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For ICC, we recommend 4% PFA fixation followed by permeabilization with ice cold methanol. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X, or fixation/permeabilization with ice cold methanol alone.
- RRID
-
AB_2910477 (BioLegend Cat. No. 602651)
AB_2910477 (BioLegend Cat. No. 602652)
Antigen Details
- Structure
- MAPK14 is a 360 amino-acid protein with a predicted molecular weight of 41 kD.
- Distribution
-
MAPK14 is widely expressed in the cytoplasm and nucleus of most tissues.
- Function
- MAPK14 is a serine/threonine kinase.
- Interaction
- MAPK14 phosphorylates myriad targets including transcription factors, other kinases, enzymes, and histone proteins.
- Biology Area
- Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Canovas B, Nebreda A. 2021. Nat Rev Mol Cell Biol. 22:346-366.
- Gene ID
- 1432 View all products for this Gene ID
- UniProt
- View information about MAPK14 on UniProt.org
Related FAQs
Other Formats
View All MAPK14 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-MAPK14 | 5A1-C5-F11 | WB,ICC,IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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