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Purified anti-mouse CCRL2 Antibody

Pricing & Availability
Clone
S21013E (See other available formats)
Regulatory Status
RUO
Other Names
C-C chemokine receptor-like 2, L-CCR, Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR), Cmkbr1l2, Ackr5
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1_S21013E_Purified_CCRL2_Antibody_060723
C57BL/6 mouse peritoneal macrophages were stained with anti-mouse F4/80 APC and purified anti-mouse CCRL2 (clone S21013E) (left), or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE.
  • 1_S21013E_Purified_CCRL2_Antibody_060723
    C57BL/6 mouse peritoneal macrophages were stained with anti-mouse F4/80 APC and purified anti-mouse CCRL2 (clone S21013E) (left), or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE.
  • 2_S21013E_Purified_CCRL2_Antibody_060723
    C57BL/6 mouse peritoneal macrophages were stained with anti-mouse CD117 APC and purified anti-mouse CCRL2 (clone S21013) (left), or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG PE.
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114002 100 µg 304€
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Description

CCRL2 (C-C chemokine receptor-like 2) is also known as Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR/Lccr). CCRL2 is a chemokine receptor-like, 7-transmembrane protein that can bind CCL19 or chemerin/RARRES2. Mouse CCRL2 is expressed by several leukocytes, including dendritic cells, macrophages, neutrophils, and microglia and is rapidly upregulated following stimulation with proinflammatory stimuli, such as LPS and TNF-α. CCRL2 plays various roles in regulating chemokine activities, e.g., by binding and internalizing excess local levels of the CCL19/MIP-3β chemokine. Alternatively, endothelial cell-expressed CCRL2 can bind & present the chemerin/RARRES2 chemoattractant to lymphoid cells that express the functional signaling receptor, CMKLR1, and promote their adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CCRL2-Transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S21013E completely blocks the reference clone BZ2E3.

RRID
AB_3068088 (BioLegend Cat. No. 114002)

Antigen Details

Structure
C-C chemokine receptor-like 2 (CCRL2) is a non-signaling seventransmembrane domain (7-TMD) receptor related to the atypical chemokine receptor (ACKR) family.
Distribution

Expressed in many leukocytes, including dendritic cells, macrophages, neutrophils, mast cells, microglia & astrocytes

Function
CC-chemokine receptor-like 2 (CCRL2) binds leukocyte chemoattractant chemerin and can regulate local levels of the attractant, but does not itself support cell migration.
Interaction
Macrophages, Dendritic Cells, Mast Cells
Ligand/Receptor
CCL19 and chemerin/RARRES2 bind to CCRL2
Molecular Family
Cytokine/Chemokine Receptors
Antigen References
  1. Monnier J, et al. 2012. J Immunol. 2012; 189:956-67.
  2. Shimada T, et al. 1998. FEBS Lett. 1998; 425:490-4
  3. Yoshimura T, et al. 2011. Exp Cell Res. 317:674-84.
  4. Zabel BA, et al. 2008. J Exp Med. 205:2207-20
Gene ID
54199 View all products for this Gene ID
UniProt
View information about CCRL2 on UniProt.org
Go To Top Version: 1    Revision Date: 06/07/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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