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Purified anti-mouse CD32 (Fcgr2) Antibody

Pricing & Availability
Clone
S17012B (See other available formats)
Regulatory Status
RUO
Other Names
Fcr-2, Fcr-3, Ly-17, LyM-1, Lym-1, fcRII, FcgRII, fcgr2b
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
S17012B_Pure_CD32_Antibody_1_111518
C57BL/6 mouse splenocytes were stained with CD19 APC and purified CD32 (clone S17012B, left) or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE.
  • S17012B_Pure_CD32_Antibody_1_111518
    C57BL/6 mouse splenocytes were stained with CD19 APC and purified CD32 (clone S17012B, left) or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE.
  • S17012B_Pure_CD32_Antibody_2_111518
    Mouse CD32-transfected Ba/F3 cells were stained with purified CD32 (clone S17012B, filled histogram) or purified rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG APC.
  • S17012B_Pure_CD32_Antibody_3_111518
    Mouse CD16-transfected Ba/F3 cells were stained with purified CD32 (clone S17012B, filled histogram) or purified rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG APC.
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156402 500 µg 216€
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Description

CD32 (Fcgr2) is a 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily. The extracellular region of CD32 consists of two Ig C-type domains that binds the Fc region from monomeric IgG with low affinity, but binds immune complexes efficiently. CD32 can mediate phagocytosis of immune complexes and modulate cell activation. CD32 is expressed by Macrophages, neutrophils, mast cells and B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD32 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Chen Q, et al. 2021. STAR Protocols. 2(1):100241. PubMed
RRID
AB_2783133 (BioLegend Cat. No. 156402)

Antigen Details

Structure
40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily; extracellular region consists of two Ig C-type domains.
Distribution

Macrophages, neutrophils, mast cells and B cells.

Function
Mediates phagocytosis of immune complexes, modulates cell activation.
Ligand/Receptor
Binds Fc from monomeric IgG with low affinity, binds immune complexes efficiently.
Cell Type
B cells, Macrophages, Mast cells, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Negishi-Koga T, et al. 2015. Nat Commun. 6:6637
  2. Yamada DH, et al. 2015. Immunity. 42:379
  3. Clatworthy MR, et al. 2014. Nat Med. 20:1458
  4. Li F and Ravetch JV. 2011. Science. 333:1030
  5. Xiang Z, et al. 2007. Nat Immunol. 8:419
Gene ID
14130 View all products for this Gene ID
UniProt
View information about CD32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/16/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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