- Clone
- D8402E8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
504702 | 500 µg | 198€ |
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The D8402E8 antibody reacts with mouse IL-9.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Baculovirus-expressed, recombinant mouse IL-9
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Capture1,2: The purified D8402E8 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated D9302C12 antibody (Cat. No. 504804) as the detecting antibody. - Application References
-
- Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Renauld, J., et al. 1995. J. Leuk. Biol. 57:353.
- Gounni, A.S.,et al.2004. J. Immunol.173:2771.J.Immunol
- Product Citations
-
- RRID
-
AB_315344 (BioLegend Cat. No. 504702)
Antigen Details
- Structure
- Cytokine; 30-40 kD (Mammalian)
- Bioactivity
- Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
- Cell Sources
- IL-2 activated TH2 lymphocytes
- Cell Targets
- T lymphocytes, erythroid precursors
- Receptors
- IL-9R
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux, V. 1992. Research Immunology 143:385.
3. Renauld, J., et al. 1993. Adv. Immunol. 54:79.
4. Yang, Y. 1992. Leuk. Lymphoma 8:441. - Regulation
- Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
- Gene ID
- 16198 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org
Related FAQs
Other Formats
View All IL-9 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse IL-9 | D8402E8 | ELISA Capture |
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