Purified anti-mouse Notch 1 Antibody anti-Notch 1

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Clone: HMN1-12 (See other available formats)
Regulatory Status: RUO
Other Names: Neurogenic locus notch homolog protein 1
Isotype: Armenian Hamster IgG
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Mouse Notch-1 transfected cells stained with purified HMN1-12, followed by anti-Armenian hamster IgG PE

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130602

500 µg

315€

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Description

The Notch receptors are highly conserved from invertebrates to mammals. Notch 1 and Notch 2 exhibit the greatest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch 3 gene mutation can cause CADASIL, an inherited early stroke syndrome.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Notch1-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_1227721 (BioLegend Cat. No. 130602)

Antigen Details

Structure: Transmembrane receptor. Heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Distribution: Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart.
Function: Regulates broad spectrum of various cell lineage commitment processes, including myogenesis, neurogenesis, gliogenesis, and lymphocyte development.
Ligand/Receptor: Jag 1,2; Delta 1,2
Cell Type: Neural Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Ehebauer ME, et al. 2006. Biochem. J. 392:13.
2. Shimizu K, et al. 2000. Mol. Cell. Biol. 20:18.
3. Tanigaki K, et al. 2007. Nature Immunol. 8:4514.
4. Kraman M, et al. 2005. FASEB J. 19:1311.
Gene ID: 18128 View all products for this Gene ID
UniProt: View information about Notch 1 on UniProt.org

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Other Formats

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Description	Clone	Applications
Purified anti-mouse Notch 1	HMN1-12	FC,IHC
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Biotin anti-mouse Notch 1	HMN1-12	FC
APC anti-mouse Notch 1	HMN1-12	FC
Brilliant Violet 421™ anti-mouse Notch 1	HMN1-12	FC
TotalSeq™-A0442 anti-mouse Notch 1	HMN1-12	PG
TotalSeq™-C0442 anti-mouse Notch 1	HMN1-12	PG
TotalSeq™-B0442 anti-mouse Notch 1 Antibody	HMN1-12	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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