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Purified anti-Nur77 Antibody

Pricing & Availability
Clone
1E10A15 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear receptor subfamily 4, group A, member 1 (NR4A1), GFRP, HMR, Testicular receptor 3 (TR3)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1E10A15_Purified_Nur77_Antibody_1_113018
Whole cell extracts (15 µg protein) from serum starved HeLa cells untreated (-) or treated (+) with 200 nM PMA for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:1000 dilution) of Purified anti-Nur77 Antibody, clone 1E10A15, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • 1E10A15_Purified_Nur77_Antibody_1_113018
    Whole cell extracts (15 µg protein) from serum starved HeLa cells untreated (-) or treated (+) with 200 nM PMA for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:1000 dilution) of Purified anti-Nur77 Antibody, clone 1E10A15, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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653501 25 µg 81€
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653502 100 µg 212€
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Description

Nur77, a transcription factor belonging to a superfamily of orphan nuclear receptors, has been shown to activate a subset of genes containing NGFI-B response element (NBRE). Nur77 expression is upregulated in response to stimuli of numerous growth factors, mitogens, and inflammatory substances, indicating that Nur77 has regulatory roles in cell growth and inflammation. Nur77 has also been shown to participate in programmed cell death through mitochondrial targeting and interaction with Bcl-2. In the endocrine system, Nur77 has been reported to participate in regulating production of steroidogenic enzymes. Nur77 and NOR-1 double knockout mice develop acute myeloid leukemia, suggesting that Nur77 plays a role in regulating differentiation of myeloid progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Nur77 recombinant protein (1-266 aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1 - 0.5 µg/mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2561762 (BioLegend Cat. No. 653501)
AB_2561762 (BioLegend Cat. No. 653502)

Antigen Details

Structure
64 kD protein containing a DNA-binding domain, two zinc finger domain, and a potential ligand-binding domain
Distribution

Nucleus

Function
Nur77 is a transcription factor that mediates various physiological stimuli, such as growth factors, hormones, inflammatory, and energy metabolic signals. Nur77 plays roles in many important biological processes including cell growth, differentiation, and apoptosis.
Interaction
Interacts with GADD45GIP1 and Bcl-2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers, Steroid Receptors/Nuclear Receptors
Antigen References

1. Nakai A, et al. 1990. Mol. Endocrinol. 4:1438.
2. Han YH, et al. 2006. Oncogene 25:2974.
3. Li M, et al. 2010. Mol. Cell. Endocrinol. 319:39.
4. Pearen MA, et al. 2010. Mol. Endocrinol. 24:1891.
5. Moll UM, et al. 2006. Oncogene 25:4725.
6. Fassett MS, et al. 2012. Proc. Natl. Acad. Sci. USA 109:3891.

Gene ID
3164 View all products for this Gene ID
UniProt
View information about Nur77 on UniProt.org
Go To Top Version: 4    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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