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Purified anti-PAR-2 Antibody (Previously Covance catalog# MMS-5116)

Pricing & Availability
Clone
SAM11 (See other available formats)
Regulatory Status
RUO
Other Names
Proteinase-activated receptor 2, thrombin receptor-like 1, G-protein coupled receptor 11, protease-activated receptor 2, coagulation factor II receptor-like 1
Previously
Covance Catalog# MMS-5116
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
SAM11_Purified_PAR-2_Antibody_1_070518
IHC staining of purified anti-PAR-2 antibody (clone SAM11) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SAM11_Purified_PAR-2_Antibody_1_070518
    IHC staining of purified anti-PAR-2 antibody (clone SAM11) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SAM11_Purified_PAR-2_Antibody_3_070518
    Western blot of purified anti-PAR-2 antibody (clone SAM11). Lane 1: Molecular weight marker; Lane 2: 10 µg of NIH3T3 cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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817201 100 µL 220€
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Description

Proteinase-activated receptor 2 (PAR-2), also known as G-protein coupled receptor 1 or thrombin receptor-like 1, is a receptor for trypsin and trypsin-like enzymes coupled to G proteins. The function of PAR-2 is mediated through the activation of multiple signaling pathways, including MAPK, phospholipase C, intracellular calcium, and I-kappaB kinase/NF-kappaB and Rho. PAR-2 is involved in the regulation of innate and adaptive immunity, the modulation of inflammatory responses, and as a sensor of proteolytic enzymes generated during the course of infection. PAR-2 is also involved in the recruitment of leukocytes to inflammation sites, promotion of dendritic cell maturation and trafficking to lymph nodes, production of superoxide, and T-cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against human PAR-2 protein and was mapped to amino acids 37-50.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P) and immunoblotting (WB).

Product Citations
  1. Ghorpade DS, et al. 2018. Nature. 32:181. PubMed
  2. Verma M, et al. 2021. J Exp Med. 218:. PubMed
RRID
AB_2564810 (BioLegend Cat. No. 817201)

Antigen Details

Structure
Expected MW: 55 kD
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Gene ID
2150 View all products for this Gene ID
UniProt
View information about PAR-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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