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Purified anti-SHP-2 Antibody

Pricing & Availability
Clone
A20016A (See other available formats)
Regulatory Status
RUO
Other Names
CFC, NS1, JMML, SHP2, BPTP3, PTP2C, METCDS, PTP-1D, SH-PTP2, SH-PTP3, Syp, Protein Tyrosine Phosphatase Non-Receptor Type 11, SH2 Domain-Containing Protein Tyrosine Phosphatase 2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A20016A_PURE_SHP-2_Antibody_091421.png
Total cell lysates (15 µg total protein) from K-562 (Lane 1), HeLa (Lane 2), HEK293 (Lane 3), and NIH/3T3 (Lane 4) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL of anti-SHP-2 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at 1:50,000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder.
  • A20016A_PURE_SHP-2_Antibody_091421.png
    Total cell lysates (15 µg total protein) from K-562 (Lane 1), HeLa (Lane 2), HEK293 (Lane 3), and NIH/3T3 (Lane 4) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL of anti-SHP-2 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at 1:50,000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder.
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948201 25 µg 95€
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948202 100 µg 235€
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Description

Src homology-2-containing protein tyrosine phosphatase 2 (SHP-2) protein, encoded by PTPN11 gene, is a well-known oncogenic protein tyrosine phosphatase. SHP-2 regulates cellular functions by dephosphorylating targets downstream of PD1, EGFRvIII, HGF receptor, and cytokine receptors. Cellular processes controlled by SHP-2 include mitotic cycle, cell growth, differentiation, and oncogenesis. SHP-2 has been recognized as a potential therapeutic target for cancers including breast cancer, lung cancer, leukemia, gastric cancer, and oral cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human SHP-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes endogenous human and mouse SHP-2 proteins in K-562, HeLa, HEK293, and NIH/3T3 cell lysates by WB.

Product Citations
  1. Taylor J, et al. 2023. Front Immunol. 14:1119350. PubMed
RRID
AB_2894536 (BioLegend Cat. No. 948201)
AB_2894536 (BioLegend Cat. No. 948202)

Antigen Details

Structure
SHP-2 is a 593 amino acid protein with a molecular mass of 68 kD.
Distribution

Cytoplasm and Nucleus

Function
SHP-2 regulates cell proliferation, apoptosis, invasion, and metastasis.
Interaction
SHP-2 interacts with PD1, EGFR, HGF receptor, RET, FGFR2, FGFR3, PI3K/AKT, and TLR3 signaling pathways.
Cell Type
Lymphocytes, T cells, Th1
Biology Area
Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Hao C, et al. 2020. Oncogene. 39:7166-7180.
  2. Marie D., et al. 2008. Cell Signal. 20:453-9.
  3. Cheng Kui QU. 2000. Cell Research. 10:279-288.
Gene ID
5781 View all products for this Gene ID
UniProt
View information about SHP-2 on UniProt.org
Go To Top Version: 1    Revision Date: 09/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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