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Purified anti-STING (TMEM173) Antibody

Pricing & Availability
Clone
O94E12 (See other available formats)
Regulatory Status
RUO
Other Names
Transmembrane Protein 173, MPYS, Endoplasmic Reticulum Interferon Stimulator, ERIS, MITA, NET23, and Mitochondrial Mediator of IRF3 Activation
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_O94E12_PURE_STING_TMEM173_Antibody_WB_112817
Western blot analysis of cell lysates from THP-1 (positive control) and HEL (low expression negative control) cells using STING Mouse primary antibody (clone O94E12, 1:500 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1_O94E12_PURE_STING_TMEM173_Antibody_WB_112817
    Western blot analysis of cell lysates from THP-1 (positive control) and HEL (low expression negative control) cells using STING Mouse primary antibody (clone O94E12, 1:500 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 2_O94E12_Purified_STING_Antibody_2_031324
    IHC staining of Purified anti-STING (TMEM173) (clone O94E12) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panels A and C) and with (panels B and D) 5 µg/mL of Purified anti-STING (TMEM173) (clone O94E12) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X (panels A and B) and 40X (panels C and D) objectives. Scale bar: 50 µm
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675902 100 µg 212€
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Description

STING (TMEM173), also called stimulator of interferon genes, is a transmembrane adaptor protein that plays an important role in the innate immune response against viral and bacterial infections. It has been shown that cyclic GMP-AMP synthase (cGAS) plays a key role in STING activation. Upon activation, STING triggers downstream transcription factors STAT6 and IRF3 through TBK1 and leads to production of IFN-β. IFN-β protects infected cells and nearby cells from local infection. It is clear that chronic STING activation is involved in chronic inflammatory diseases, such as autoimmune syndromes and cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant STING (TMEM173) protein produced in 293T cells.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/ml (1:250-1:500). For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 2 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Serrano R, et al. 2022. Oncoimmunology. 11:2030021. PubMed
  2. Kabelitz D, et al. 2022. Sci Rep. 12:17827. PubMed
RRID
AB_2565608 (BioLegend Cat. No. 675902)

Antigen Details

Structure
A 379 amino acid protein with a predicated molecular weight around 42 kD, and observed molecular weight on SDS gel is around 35 kD.
Distribution

STING is expressed in various hematopoietic cells such as T lymphocytes, NK cells, myeloid cells and monocytes and is also highly expressed in lung, ovary, heart, smooth muscle, retina, bone marrow and vagina.

Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response.
Interaction
MHC-II complex, DDX58/RIG-I, MAVS, SSR2, RNF5, TRIM56, TBK1, IFIT1 and IFIT2.
Cell Type
B cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References

1. Lemos H, et al. 2015. Expert Rev Clin Immunol. 11:155.
2. Konno H, et al. 2014. Microbes Infect. 16:998.
3. Maringer K, et al. 2014. Cytokine Growth Factor Rev. 25:669.
4. Cai X, et al. 2014. Mol Cell. 54:289.
5. Barber GN, et al. 2014. Trends Immunol. 35:88.
6. Sun L, et al. 2013. Science. 339:786.

Gene ID
340061 View all products for this Gene ID
UniProt
View information about STING on UniProt.org
Go To Top Version: 4    Revision Date: 03/13/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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