- Clone
- BL26882 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
688202 | 25 µg | 72€ |
The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic Peptide
- Formulation
- PBS pH: 7.4, containing 0.5 % BSA, 0.02% sodium azide, and 50% glycerol.
- Preparation
- The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2629594 (BioLegend Cat. No. 688202)
Antigen Details
- Structure
- The amino acid sequence of the Strep-tag is: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys.
- Function
- Strep-tag can be attached to the N- or C-terminus of protein to become Strep-tag fused protein.
- Biology Area
- Cell Biology
- Antigen References
-
1. Skerra A, et al. 2000. Methods Enzymol. 326:271.
2. Schmidt TG, et al. 2007. Nat. Protoc. 2:1528. - Gene ID
- NA
- UniProt
- View information about Strep-Tag on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Strep-Tag Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Strep-Tag | BL26882 | WB |
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Purified anti-Strep-Tag
Recombinant Strep tag fused human Her2 (23-652 aa) was reso...
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