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Purified anti-TATA Binding Protein Antibody

Pricing & Availability
Clone
W22002D (See other available formats)
Regulatory Status
RUO
Other Names
TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TBP
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W22002D_Purified_TATA-Binding-Protein_WB_050824
Whole cell extracts (15 µg total protein per lane) from indicated human cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-TATA Binding Protein (clone W22002D) overnight at 4°C (left blot). For comparison, we probed a separate membrane (right blot) containing the same human lysates with another antibody on the market, Purified anti-TATA Binding Protein (clone 1TBP18). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W22002D_Purified_TATA-Binding-Protein_WB_050824
    Whole cell extracts (15 µg total protein per lane) from indicated human cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-TATA Binding Protein (clone W22002D) overnight at 4°C (left blot). For comparison, we probed a separate membrane (right blot) containing the same human lysates with another antibody on the market, Purified anti-TATA Binding Protein (clone 1TBP18). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W22002D_Purified_TATA-Binding-Protein_IP_040124
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified rat IgG2a, κ isotype control (Cat. No. 400502) or Purified anti-TATA Binding Protein (clone W22002D). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a control antibody (clone 1TBP18) (Cat. No. 668306) against a separate epitope of anti-TATA Binding Protein. Lane M: Molecular weight marker
  • C. W22002D_Purified_TATA-Binding-Protein_ICC-1_040124
    HCT 116 cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-TATA Binding Protein (clone W22002D) (panel A), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panel C). Scale bar: 50 µm
  • D. W22002D_Purified_TATA-Binding-Protein_ICC-2_040124
    Hep G2 cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-TATA Binding Protein (clone W22002D) (panel A), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged (panel C). Scale bar: 50 µm
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634251 25 µg 140€
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634252 100 µg 348€
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Description

The TATA-binding protein, also known as TBP or Tata Binding Protein, is the general transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), which plays a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This protein is a component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription. The rate of PIC formation is assumed to be primarily dependent on the rate of the association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human TATA Binding Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For the use of this antibody (clone W22002D) in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 100% ice-cold methanol.

This product does not cross-react with mouse or rat.

This product is not suitable for use in immunohistochemistry on formalin-fixed paraffin-embedded tissues. 

RRID
AB_3106159 (BioLegend Cat. No. 634251)
AB_3106159 (BioLegend Cat. No. 634252)

Antigen Details

Structure
TBP is composed of 339 amino acids with a predicted molecular weight of 37 kD.
Distribution

Nucleus

Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Interaction
Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3, SPIB, SNAPC1, SNAPC2 and SNAPC4, UTF1, BRF2, UBTF, GPBP1, HIV-1 Tat, herpes simplex virus 1 ICP4, TBP-TATA complex, LLPH, HSF1.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Hoffmann A, et al. 1990. Nature. 346:387-90.
  2. Peterson MG, et al. 1990. Science. 248:1625-30.
  3. Kwon H, et al. 1994. J Biol Chem. 269:30140-6.
  4. Comai L, et al. 1994. Science. 266:1966-72.
Gene ID
6908 View all products for this Gene ID
UniProt
View information about TATA on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/01/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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