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Purified anti-TBLR1 Antibody

Pricing & Availability
Clone
4F3-A8-D9 (See other available formats)
Regulatory Status
RUO
Other Names
Transducin Beta Like 1 X-Linked Receptor 1, F-Box-Like/WD Repeat-Containing Protein TBL1XR1, Transducin Beta-Like 1X-Related Protein 1, MRD41, DC42
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4F3-A8-D9_PURE_TBLR1_Antibody_1_120219
Western blot of purified anti-TBLR1 antibody (clone 4F3-A8-D9). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain nuclear lysate; Lane 3: 20 µg of rat brain nuclear lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F3-A8-D9_PURE_TBLR1_Antibody_1_120219
    Western blot of purified anti-TBLR1 antibody (clone 4F3-A8-D9). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain nuclear lysate; Lane 3: 20 µg of rat brain nuclear lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F3-A8-D9_PURE_TBLR1_Antibody_2_120219
    IHC staining of purified anti-TBLR1 antibody (clone 4F3-A8-D9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4F3-A8-D9_PURE_TBLR1_Antibody_3_120219
    IHC staining of purified anti-TBLR1 antibody (clone 4F3-A8-D9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 10.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
870001 25 µg 85€
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870002 100 µg 221€
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Description

TBLR1 is a member of the WD40 repeat-containing family of proteins. It shares similarity with TBL1 in sequence, and is believed to be a component of the complex of nuclear corepressor and HDAC3. TBLR1 is involved in signaling pathway during development through the regulation of chromatin acetylation. It is also associated with diseases including pierpont syndrome, mental retardation, and autism spectrum disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human TBLR1 protein fragments expressed in E. coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 0.5 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820184 (BioLegend Cat. No. 870001)
AB_2820184 (BioLegend Cat. No. 870002)

Antigen Details

Structure
TBLR1 is a 514 amino acid protein with a predicted and observed molecular mass of ~56 kD.
Distribution

Tissue Distribution: Brain, immune system, heart, and lung
Cellular Distribution: Nucleus

Function
Chromatin acetylation
Interaction
HDAC3, TBL1, NCOR1, NCOR2, MECP2
Cell Type
B cells, Monocytes, Neurons, NK cells
Biology Area
Cancer Biomarkers, Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family
Nuclear Markers
Antigen References
  1. Xi X, et al. 2019. Eur J Pharmacol. 844:259-267.
  2. Nishi A, et al. 2017. Sci Rep. 7(1):2887.
Gene ID
81004 View all products for this Gene ID 365755 View all products for this Gene ID
UniProt
View information about TBLR1 on UniProt.org
Go To Top Version: 1    Revision Date: 12/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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