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Purified anti-Testosterone Antibody

Pricing & Availability
Clone
3T16 (See other available formats)
Regulatory Status
RUO
Other Names
Testosterone
Isotype
Mouse IgG2b, κ
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Product Citations
publications
3T16_Pure_Testosterone_Antibody_1_081618
Capture ELISA of purified anti- Testosterone antibody (clone 3T16) and isotype-matched control mouse IgG1 binding to OVA-conjugated Testosterone (OVA-Testosterone). ELISA was performed by coating wells with 100 ng of clone 3T16. The wells were then incubated at 37°C for 1 hour with serially diluted (0.002 to 2 µg/ml) OVA-Testosterone, followed by incubation with rabbit anti-OVA antibody, and horseradish peroxidase labeled goat anti-Rabbit secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • 3T16_Pure_Testosterone_Antibody_1_081618
    Capture ELISA of purified anti- Testosterone antibody (clone 3T16) and isotype-matched control mouse IgG1 binding to OVA-conjugated Testosterone (OVA-Testosterone). ELISA was performed by coating wells with 100 ng of clone 3T16. The wells were then incubated at 37°C for 1 hour with serially diluted (0.002 to 2 µg/ml) OVA-Testosterone, followed by incubation with rabbit anti-OVA antibody, and horseradish peroxidase labeled goat anti-Rabbit secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • 3T16_Pure_Testosterone_Antibody_2_081618
    Direct ELISA of purified anti-Testosterone antibody (clone 3T16) and isotype-matched control mouse IgG1 binding to plate-immobilized OVA-Testosterone. ELISA was performed by coating wells with 600 ng OVA-Testosterone. The wells were then incubated with clone 3T16 or mouse IgG at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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854001 25 µg 85€
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854002 100 µg 221€
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Description

In vertebrate, Testosterone is the major male sex hormone that is essential to the development of reproductive organs and secondary sexual characteristics. It also plays a key role in muscle development, body mass increase and body fair growth. Importantly, it is critical to sperm development.

In vivo, testosterone is primarily secreted by testicles (in male), and by ovaries (in female), and eventually metabolized in liver. In the central nervous system, Testosterone is one of the key components of the hypothalamic-pituitary-adrenal axis, which is activated acutely by dominance challenger. It has been reported that different level of sex hormones, including testosterone, determines human behavior, as well as brain development and recovery after injury.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Testosterone-3-(O-carboxymethyl)oxime coupled to bovine serum albumin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 10 - 150 ng/mL is recommended. For Direct ELISA applications, a concentration range of 1.0 - 10 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody has been tested against human and mouse sera.

Application References
  1. Fantl VE, et al. 1983. J Steroid Biochem. 19(5):1605 (PubMed)
RRID
AB_2750291 (BioLegend Cat. No. 854001)
AB_2750291 (BioLegend Cat. No. 854002)

Antigen Details

Structure
Testosterone is an Androgen with a molecular mass of 288.431 g/mol.
Distribution

Tissue Source: Primarily in reproductive organs (Testis, ovaries), trace amount in other organs including brain and liver.
Cellular Source: Secreted molecule.

Ligand/Receptor
Binds to androgen receptors.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Antigen References
  1. Dong DX & Ji ZG. 2017. Chin Med J (Engl). 130:2991
  2. Roselli CE. 2017. J Neuroendocrinol. Doi:10.1111/jne.12562
  3. Mohammad OS, et al. 2017. Cancers (Basel). 9(12).pii:E166
Gene ID
NA
UniProt
View information about Testosterone on UniProt.org
Go To Top Version: 1    Revision Date: 08/16/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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