- Clone
- IIB1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
668501 | 100 µg | 259€ |
TFIIB is one of the ubiquitous transcription factors required for transcription initiation by RNA polymerase II. It plays an important role in pre-initiation complex assembly, which provides a bridge between promoter-bound TFIID and RNA polymerase II. Moreover, TFIIB also directly modulates the catalytic center of RNA polymerase II.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein.
- Formulation
- Phosphate-buffered solution, pH 7.2.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
ICC - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone IIB1 recognizes epitope 1-51.
- Application References
-
- Thompson NE, et al. 1995. J. Biol. Chem. 270:4735. (IP)
- RRID
-
AB_2564439 (BioLegend Cat. No. 668501)
Antigen Details
- Structure
- 316 amino acids with a predicted molecular weight of approximately 35 kD.
- Distribution
-
Nucleus.
- Function
- The Transcription factor is required for transcription initiation by RNA polymerase II.
- Interaction
- Transcription factors IID and IIA and RNA polymerase II.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Ha I, et al. 1991. Nature 352:689.
3. Hurwitz, J. 2005. J. Biol. Chem. 280:42477.
4. Deng W, Roberts SG. 2007. Chromosoma. 5:417.
5. Hirata A, et al. 2008. Nature 451:851. - Gene ID
- 2959 View all products for this Gene ID
- UniProt
- View information about TFIIB on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All TFIIB Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TFIIB | IIB1 | WB,ICC,IP |
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