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Purified anti-ZO-3 Antibody

Pricing & Availability
Clone
W22143C (See other available formats)
Regulatory Status
RUO
Other Names
Tight Junction Protein ZO-3, Zonula Occludens protein 3, Zona Occludens Protein 3, ZO3, Zona Occludens 3
Isotype
Rat IgG2a, κ
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Product Citations
publications
A. W22143C_PURE_ZO-3_WB_041924
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved in 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-ZO-3 (clone W22143C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • A. W22143C_PURE_ZO-3_WB_041924
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved in 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-ZO-3 (clone W22143C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • B. W22143C_PURE_ZO-3_ICC_041924
    LN-319 cells (negative cell line, panel A) and MCF-7 cells (positive cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with 0.5% Triton X-100, and blocked with 5% FBS. The cells were stained with Purified anti-ZO-3 (clone W22143C) overnight at 4°C, followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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637651 25 µg 156€
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637652 100 µg 424€
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Description

ZO-3 is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family. Together with ZO-1 and ZO-2, they act as scaffold proteins that link transmembrane proteins in the tight junctions such as claudins, junctional adhesins and occludins to the actin cytoskeleton forming a boundary between the apical and basolateral plasma membrane on epithelial cells. The importance of this complex of proteins is to maintain tight junctions and limit the paracellular movement of substances. In addition, ZO-3 sequesters Cyclin D1 avoiding its degradation during mitosis and enabling S-phase transition. Celiac Disease 1 has been associated with ZO-3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant TJP3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3106184 (BioLegend Cat. No. 637651)
AB_3106184 (BioLegend Cat. No. 637652)

Antigen Details

Structure
ZO-3 is a 130 kDa protein with multiple PDZ motifs, one SH3 domain, and one guanylate kinase-like domain.
Distribution

Tight junctions in the cytoplasmic side of the plasma membrane, nucleus

Function
ZO-3 is part of the protein complex that maintains the boundary between the apical and basolateral plasma membrane domains of epithelial and endothelial cells. This complex of proteins also limits movement of substances through the paracellular space.
Interaction
ZO-1, ZO-2, Claudins, transmembrane adhesins, actin cytoskeleton, Cyclin D1
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Cell Structure
Molecular Family
Adaptor Proteins, Adhesion Molecules, Gap Junction Proteins, Scaffold Proteins
Antigen References
  1. Haskins J, et al. 1998. J Cell Biol. 141:199-208
  2. Adachi M, et al. 2006. Mol Cell Biol. 26:9003-9015.
  3. Itoh M, et al. 1999. J Cell Biol. 147:1351-1363.
  4. Zhuang X, et al. 2023. Biomedicines. 11:3136.
Gene ID
27134 View all products for this Gene ID
UniProt
View information about ZO-3 on UniProt.org
Go To Top Version: 1    Revision Date: 04/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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