- Clone
- HTK888 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_HTK888_Purified_IgG_Antibody_021920 1_HTK888_Purified_IgG_Antibody_021920](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HTK888_Purified_IgG_Antibody_021920.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (190 µg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 µg of purified Armenian Hamster IgG isotype ctrl antibody (clone HTK888) (Cat. No. 400902) or purified anti-mouse/human Helios antibody (clone 22F6) (Cat. No. 137202). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit antibody against human Helios overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG (minimal x-reactivity) antibody (Cat. No. 406401) at a 1:3000 dilution. Lane M: Molecular weight marker. -
Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from Jurkat cells. ChIP was performed with 3 µg of chromatin and either 1.2 µg of Go-ChIP-Grade™ Purified Armenian Hamster IgG Isotype Control Antibody (clone HTK888, in black) or no antibody (beads control, in gray). Protein G beads were used to isolate immune complexes. The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for active (human GAPDH promoter and human RPL30 exon 3) and inactive (human α-Satellite repeats) loci. The amount of immunoprecipitated DNA in each sample is represented as percentage to the total amount of input chromatin, which is equivalent to 100%.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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400901 | 50 µg | 38€ | ||||
400902 | 500 µg | 104€ |
This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Trinitrophenol + KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The immunoglobulin was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC, ICFC - Quality tested
IP, ChIP - Verified
ICC, IHC, WB, FA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test.
- Application Notes
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Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg). - Application References
- Product Citations
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- RRID
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AB_3096169 (BioLegend Cat. No. 400901)
AB_3096169 (BioLegend Cat. No. 400902)
Antigen Details
- Gene ID
- NA
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Armenian Hamster IgG Isotype Ctrl
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Biotin Armenian Hamster IgG Isotype Ctrl
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FITC Armenian Hamster IgG Isotype Ctrl
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PE Armenian Hamster IgG Isotype Ctrl
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PE/Cyanine5 Armenian Hamster IgG Isotype Ctrl
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Purified Armenian Hamster IgG Isotype Ctrl
Whole cell extracts (190 µg total protein) prepared from Jur... Chromatin Immunoprecipitations (ChIP) were done with fixed a... -
PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 488 Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 647 Armenian Hamster IgG Isotype Ctrl
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Pacific Blue™ Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 700 Armenian Hamster IgG Isotype Ctrl
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APC/Cyanine7 Armenian Hamster IgG Isotype Ctrl
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PerCP Armenian Hamster IgG Isotype Ctrl
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PerCP/Cyanine5.5 Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 570™ Armenian Hamster IgG Isotype Ctrl
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Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 510™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 605™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 650™ Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 785™ Armenian Hamster IgG Isotype Ctrl
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PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl
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Alexa Fluor® 594 Armenian Hamster IgG Isotype Ctrl
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APC/Fire™ 750 Armenian Hamster IgG Isotype Ctrl
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Brilliant Violet 711™ Armenian Hamster IgG Isotype Ctrl
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GoInVivo™ Purified Armenian Hamster IgG Isotype Ctrl
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TotalSeq™-A0241 Armenian Hamster IgG Isotype Ctrl
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KIRAVIA Blue 520™ Armenian Hamster IgG Isotype Ctrl
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TotalSeq™-C0241 Armenian Hamster IgG Isotype Ctrl
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TotalSeq™-B0241 Armenian Hamster IgG Isotype Ctrl
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Spark YG™ 570 Armenian Hamster IgG Isotype Ctrl
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Spark Red™ 718 Armenian Hamster IgG Isotype Ctrl
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Spark Blue™ 515 Armenian Hamster IgG Isotype Ctrl
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PerCP/Fire™ 780 Armenian Hamster IgG Isotype Ctrl
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Spark Blue™ 574 Armenian Hamster IgG Isotype Ctrl
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Spark PLUS UV™ 395 Armenian Hamster IgG Isotype Ctrl
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