Recombinant Mouse METRNL (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Meteorin-like protein, MTL, Cometin, Subfatin, Interleukin 41, IL-41, Meteorin, Glial cell differentiation regulator-like
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Product Citations
publications
Mouse_METRNL_RECOM_CF_1_060517
Immobilized recombinant mouse METRNL induces adhesion of C6 glioma cells in a dose dependent manner with ED50 of 0.5 – 2.0 µg/mL.
  • Mouse_METRNL_RECOM_CF_1_060517
    Immobilized recombinant mouse METRNL induces adhesion of C6 glioma cells in a dose dependent manner with ED50 of 0.5 – 2.0 µg/mL.
  • Mouse_METRNL_RECOM_CF_2_012122
    Immobilized recombinant mouse METRNL at 1.1 µg/ml enhances neurite outgrowth from rat E18 cortical neurons.
  • Mouse_METRNL_RECOM_CF_3_012122
    Rat E18 cortical neurons treated with PBS (negative control).
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764704 25 µg 300€
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764706 100 µg 877€
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Description

METRNL, a secreted protein homologous to Meteorin, shows high levels of expression in white adipose tissue and barrier tissues.  Low expression of METRNL is observed in the adult mouse brain and it is expressed in restricted sites of the brain during development.  Based on its expression and distinct functions, METRNL is also called Cometin, Subfatin, and Interleukin 39.  METRNL is induced following exercise or cold exposure that promotes energy expenditure.  It stimulates energy expenditure associated with the browning of the white fat adipose tissues and improves glucose tolerance.  METRNL stimulates an eosinophil-dependent increase in IL-4 expression and promotes alternative activation of adipose tissue macrophages.  METRNL is also involved in neural development.  Jorgensen et al first described METRNL  protein and demonstrated its function as a neurotrophic factor similar to Meteorin.  METRNL was shown to be a latent process gene involved in neurite extension.  BioLegend in-house testing shows METRNL can induce C6 glioma cell adhesion and also enhance neurite outgrowth from cultured mouse cortical neurons.  

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse METRNL, amino acids Gln46-Glu311 (Accession # NP_659046.1) with C-terminal His10 was expressed in CHO cells.
Molecular Mass
The 279 amino acid recombinant protein has a predicted molecular mass of approximately 31.4 kD. The DTT-reduced protein migrates at approximately 37 kD, and non-reduced proteins migrate at approximately 36 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 1.0 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50=0.5 - 2.0 µg/mL as measured by the ability of immobilized protein to support the adhesion of C6 glioma cells. Calcein-AM was used to measure the number of adherent cells.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

High levels of expression in white adipose tissue and barrier tissues.  Low level of expression in adult mouse brain.

Function
Stimulates energy expenditure associated with the browning of the white fat adipose tissues, promotes alternative activation of adipose tissue macrophages, involved in neurite extension.
Interaction
Eosinophils, neurons
Bioactivity
Measured by the ability of immobilized protein to support the adhesion of C6 glioma cells.
Biology Area
Neuroscience
Antigen References

1. Watanabe K, et al. 2012. J. Cell Sci. 125:2198-211.
2. Jorgensen JR, et al. 2012. Exp. Neurol. 233:172-81
3. Li Zy, et al. 2014. Biochem. Biophys. Res. Comm. S0006-291X(13)01148-0.
4. Rao RR, et al. 2014. Cell 157:1279-91.
5. Ushach I, et al. 2015. Clin. Immunol. 156:119-27.
6. Gong W, et al. 2016. PLoS One 11(10):e0164446.
7. Löffler D, et al. 2017. Int. J. Obes. (Lond). 41:112-119.

Gene ID
210029 View all products for this Gene ID
UniProt
View information about METRNL on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 0    Revision Date: 06/06/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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