- Clone
- IP26 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α/β TCR, TCR α/β
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- Application References
Antigen Details
- Structure
- Ig superfamily, with CD3 forms CD3/TCR complex
- Distribution
-
T cells, thymocytes
- Function
- Antigen recognition, T cell activation
- Ligand/Receptor
- Peptide bound to MHC
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.
- Gene ID
- 6955 View all products for this Gene ID 6957 View all products for this Gene ID
- UniProt
- View information about TCR alpha/beta on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All TCR α/β Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human TCR α/β
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 FITC -
PE anti-human α/β T Cell Receptor
Human peripheral blood lymphocytes stained with IP26 PE -
PE/Cyanine5 anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 PE/Cyan... -
Purified anti-human TCR α/β
Human peripheral blood lymphocytes stained with purified IP2... -
PE/Cyanine7 anti-human TCR α/β
Human peripheral blood lymphocytes stained with CD19 (HIB19)... -
Alexa Fluor® 488 anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 Alexa F... -
Alexa Fluor® 647 anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 Alexa F... -
Pacific Blue™ anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 Pacific... -
APC anti-human TCR α/β
Human peripheral blood lymphocytes stained with IP26 APC -
Brilliant Violet 421™ anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Dazzle™ 594 anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Cyanine7 anti-human TCR α/β
Human peripheral blood lymphocytes were stained with TCR a/ß... -
Alexa Fluor® 700 anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 510™ anti-human TCR α/β
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC/Fire™ 750 anti-human TCR α/β
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 605™ anti-human TCR α/β
Human peripheral blood lymphocytes were stained with CD3 FIT... -
TotalSeq™-A0224 anti-human TCR α/β
-
Brilliant Violet 711™ anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 785™ anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-C0224 anti-human TCR α/β
-
Brilliant Violet 750™ anti-human TCR α/β
Human peripheral blood lymphocytes were stained with TCRα/β ... -
TotalSeq™-B0224 anti-human TCR α/β
-
PE anti-human TCR α/β
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 810 anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0224 anti-human TCR α/β
-
GMP PE anti-human TCR α/β
Typical results from human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human TCR α/β (Flexi-Fluor™)
-
Brilliant Violet 650™ anti-human TCR α/β
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human TCR α/β
Typical results from human peripheral blood lymphocytes stai...
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