- Clone
- M-T271 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V 5T CD27.03
- Other Names
- S152, T14, TNFRSF7
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD27 (clone M-T271) StarBright UltraViolet 575 (left) or stained with anti-human CD3 (clone UCHT1) FITC only (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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356444 | 100 tests | 300€ |
This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. Contact your local field representative to order this product in a StarBright panel today.
This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.
CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human T cells from a T-ALL patient.
- Formulation
- Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 575 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* StarBright UltraViolet 575 has a maximum excitation of 340 nm and a maximum emission of 569 nm.
StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc. - Excitation Laser
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Ultraviolet Laser (355 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.
- Application References
-
- Ma S, et al. 2011. J. Virol. 85:165. (IHC)
- Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
- Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
Antigen Details
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
- Distribution
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Medullary thymocytes, T and B cell subsets, NK cells
- Interaction
- CD27 binds to CD70
- Ligand/Receptor
- CD70
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627. - Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
Related FAQs
Other Formats
View All CD27 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD27
Human peripheral blood lymphocytes were stained with purifie... -
FITC anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PE anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... Human peripheral blood was stained with CD27 (clone M-T271) ... -
PerCP/Cyanine5.5 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
APC anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PE/Cyanine7 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Pacific Blue™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Alexa Fluor® 700 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Brilliant Violet 421™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Brilliant Violet 510™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PE/Dazzle™ 594 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
APC/Cyanine7 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Biotin anti-human CD27
Human peripheral blood lymphocytes were stained with biotiny... -
APC/Fire™ 750 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Brilliant Violet 711™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
PerCP anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
Alexa Fluor® 647 anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (c... -
KIRAVIA Blue 520™ anti-human CD27
Human Peripheral blood lymphocytes were stained with CD3 APC... -
PE/Cyanine5 anti-human CD27
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine7 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
PE anti-human CD27
Typical result from human peripheral blood lymphocytes stain... -
FITC anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 650™ anti-human CD27
Human peripheral blood lymphocytes were stained with anti-hu... -
Pacific Blue™ anti-human CD27
Typical result from human peripheral lymphocytes stained eit... -
Spark Violet™ 423 anti-human CD27
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Cyanine7 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
Spark Red™ 718 anti-human CD27 (Flexi-Fluor™)
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GMP PerCP/Cyanine5.5 anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD27
Typical result from human peripheral blood lymphocytes stain... -
GMP APC anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD27
Typical results from human peripheral blood lymphocytes stai... -
StarBright UltraViolet 575 anti-human CD27
Human peripheral blood lymphocytes were stained with anti-hu...
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