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Ultra-LEAF™ Purified anti-CCL15 Antibody

Pricing & Availability
Clone
A15015A (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage inflammatory protein 1 (MIP1ž„), Macrophage inflammatory protein 5 (MIP5), Leukotactin 1 (LKN1), Hemofiltrate chemokine 2 (HCC-2), New CC chemokine 3 (NCC3)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A15015A_ULEAF_PURE_CCL15_Antibody_FA_111116
Recombinant human CCL15 chemoattracts Baf3-hCCR1 transfectants in a dose dependent manner (purple circles). Anti-human CCL15 antibody (clone A15015A, purple squares) neutralizes the chemoattraction of Baf3-hCCR1 transfectants induced by recombinant human CCL15 (6 ng/ml). The ND50 = 0.05 - 0.25 µg/ml.
  • A15015A_ULEAF_PURE_CCL15_Antibody_FA_111116
    Recombinant human CCL15 chemoattracts Baf3-hCCR1 transfectants in a dose dependent manner (purple circles). Anti-human CCL15 antibody (clone A15015A, purple squares) neutralizes the chemoattraction of Baf3-hCCR1 transfectants induced by recombinant human CCL15 (6 ng/ml). The ND50 = 0.05 - 0.25 µg/ml.
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692703 100 µg 216€
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692704 1 mg 572€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CCL15 was discovered by analyzing the upstream region of the CCL14 gene. CCL15 is arranged in tandem with CCL14 on human chromosome 17. CCL15, CCL6, CCL9, and CCL23 have an extended N-terminal domain upstream of the first two conserved chemokine cysteines. This domain is encoded by a different exon and includes 16 to 20 residues. CCL15 (1-92) is processed by cathepsin G to produce highly active forms of CCL15 [(24-92), (27-92) and (29-92)], and elastase processes CCL15 (1-92) predominantly to CCL15 (22-92). Also, CCL15 is cleaved by MT6-MMP/MMP25 to produce CCL15 with very high activity. MMP25 is localized primarily in neutrophil gelatinase granules, and it is released by neutrophil activation and degranulation in inflammatory reactions. High levels of CCL15 were detected in human renal cell carcinoma bone metastasis, and in vitro studies showed that CCL15 stimulates chemotaxis of osteoclast progenitors and enhances their differentiation in response to RANKL. CCL15 is abundant in bronchoalveolar lavage of patients with pulmonary sarcoidosis. Also, it is elevated in the serum of patients with severe asthma, and it is reduced by omalizumab, a humanized anti-IgE antibody that has been use in the treatment of asthma. It has been suggested that airway smooth muscle cells and basophils produce CCL15, and it might have an important role in asthma severity and persistence. In addition, high levels of CCL15 have been detected in the serum of patients with hepatocellular carcinoma, and human hepatic carcinoma cells express CCR1 and CCL15.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL15.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking. The ND50 is 0.05 - 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632767 (BioLegend Cat. No. 692703)
AB_2632767 (BioLegend Cat. No. 692704)

Antigen Details

Distribution

CCL15 is produced by smooth muscle cells, basophils, and hepatic carcinoma cells.

Function
CCL15 induces migration of monocytes, eosinophils, and lymphocytes. CCL15 is activated by cathepsin G, elastase, and MMPs. TNFα and IFNγ synergistically induce CCL15 in human airway smooth muscle cells in culture.
Interaction
Monocytes, eosinophils, airway smooth muscle cells, hepatic carcinoma cells, human osteogenic sarcoma cells, and osteoclast progenitors.
Ligand/Receptor
CCR1, CCR3.
Cell Type
Basophils
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pardigol A, et al. 1998. Proc. Natl. Acad. Sci. USA 95:6308.
2. Richter R, et al. 2005. J. Immunol. 175:1599.
3. Berahovich RD, et al. 2005. J. Immunol. 174:7341.
4. Shimizu Y, et al. 2011. J. Biol. Regul. Homeost. Agents 25:177.
5. Starr AE, et al. 2012. J. Biol. Chem. 287:13382.
6. Weber KL, et al. 2012. PLoS One 7:e40799.
7. Li Y, et al. 2013. Br. J. Cancer 108:99.

Gene ID
6359 View all products for this Gene ID
UniProt
View information about CCL15 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 01/19/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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