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Ultra-LEAF™ Purified anti-human CD178 (FasL) Antibody

Pricing & Availability
Clone
W19403O (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor ligand superfamily, member 6 (TNFSF6), Apoptosis Antigen Ligand 1 (APT1LG1), apoptosis antigen Ligand, CD95 ligand (CD95L), Apo-1 ligand, CD178, Fas ligand
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Recombinant human FASL (TNFSF6) (Cat. No. 589402) (black circles) induces apoptotic cell death on Jurkat cells. Ultra-LEAF™ purified anti-human CD178 (Fas-L) (clone W19403O) (purple squares) inhibits the effect of recombinant human FASL (TNFSF6) at 0.02 µg /mL on apoptotic cell death of Jurkat cells in a dose- dependent manner, whereas the GoInVivo™ purified mouse IgG2b, κ isotype control (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 5 - 25 ng/mL.
  • Recombinant human FASL (TNFSF6) (Cat. No. 589402) (black circles) induces apoptotic cell death on Jurkat cells. Ultra-LEAF™ purified anti-human CD178 (Fas-L) (clone W19403O) (purple squares) inhibits the effect of recombinant human FASL (TNFSF6) at 0.02 µg /mL on apoptotic cell death of Jurkat cells in a dose- dependent manner, whereas the GoInVivo™ purified mouse IgG2b, κ isotype control (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 5 - 25 ng/mL.
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949103 100 µg 199€
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949104 1 mg 612€
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Description

FASL was initially cloned and purified from a cytotoxic T cell hybridoma, PC60-d10S. FASL is a type II transmembrane glycoprotein of approximately 40 kD and belongs to the TNF family of membrane-associated cytokines. A soluble fragment of FASL (sFASL, 26-29 kD) has been described using in vitro proteolytic assays, and MMP7 was proposed to participate in this process. Nevertheless, TIMPs (tissue inhibitors of metalloproteinases) did not alter FASL shedding.

 

FASL is proteolytically cleaved by ADAM10, and it is the major protease responsible for FASL cleavage in murine fibroblasts and human T cells. The cleavage site is between Ser126 and Leu127. This site is outside of the self-assembly (SA) domain that allows sFASL to form trimers. It has been suggested that FASL performs its biological activity as a homotrimer. Shedding of FASL modulates FASL/FAS dependent apoptosis and affects activation-induced cell death (AICD) in a superantigen stimulation model. It has been published that sFASL has proapoptotic and antiapoptotic properties, depending on cell type and cell microenvironment.

 

sFASL can be detected in the serum of patients with dysregulated inflammatory diseases. Mutations in human FAS and FASL are associated to autoimmune lymphoproliferative syndrome (ALPS). In these patients, the homeostasis of T and B lymphocytes is disturbed, leading to hepatosplenomegaly and lymphadenopathy. Dysregulation of FAS/FASL has been connected to multiple diseases such as osteoarthritis, pulmonary fibrosis, diabetic polyneuropathy, acute coronary syndrome, bladder and gastric cancer among others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human FASL (TNFSF6)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing apoptotic cell death induced by recombinant human FASL (TNFSF6) (Cat. No. 589402) at 20 ng /mL on Jurkat cells. ND50 range: 5-25 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2936681 (BioLegend Cat. No. 949103)
AB_2936681 (BioLegend Cat. No. 949104)

Antigen Details

Structure
Homo trimeric
Distribution

Cytotoxic lymphocytes (CTL), NK cells, neurons, astrocytes, and T cells

Function
FASL induces apoptotic cell death. It plays a key role in the immune system homeostasis, cytotoxic T cell (CTL)-mediated killing of virally infected or transformed cells, NK cell-mediated death cell, and termination of immune responses by induction of activation-induced cell death. ADAM10 modulates FASL-mediated cell death.
Interaction
Activate T and B cells, myeloid cells, thymocytes, fibroblasts, splenocytes, hepatocytes, variety of cell types
Ligand/Receptor
FAS (CD95)
Cell Type
Astrocytes, Lymphocytes, Neurons, NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Immunology, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Suda T, et al. 1993. Cell. 75:1169-78.
  2. Mitsiades N, et al. 2001. Cancer Res. 61:577-81.
  3. Bodmer J, et al. 2002. Trends Biochem Sci. 27:19-26.
  4. Grassi F, et al. 2004. Arthritis Rheum. 50:498-506.
  5. Bühling F, et al. 2005. Respir Res. 6:37.
  6. Schulte M, et al. 2007. Cell Death Differ. 14:1040-9.
  7. Stranges PB, et al. 2007. Immunity. 26:629-41.
  8. Voss M, et al. 2008. Cell Commun Signal. 6:11.
  9. Dowdell KC, et al. 2010. Blood. 115:5164-9.
  10. Gorbachev AV and Fairchild RL. 2010. Eur J Immunol. 40:2006-15.
Gene ID
356 View all products for this Gene ID
UniProt
View information about CD178 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 11/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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