- Clone
- 2D10 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI CD80.1
- Other Names
- B7-1, B7, BB1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
305245 | 100 µg | 176€ | ||||
305246 | 1 mg | 572€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IP, WB, IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).
- Application References
-
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
- Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
- Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
- Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
- Wen T, et al. 2014. J Immunol. 192:5481. PubMed
- RRID
-
AB_2814138 (BioLegend Cat. No. 305245)
AB_2814138 (BioLegend Cat. No. 305246)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 60 kD
- Distribution
-
Activated B cells and T cells, macrophages, and dendritic cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561. - Gene ID
- 941 View all products for this Gene ID
- UniProt
- View information about CD80 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD80 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-human CD80 | 2D10 | FC |
FITC anti-human CD80 | 2D10 | FC |
PE anti-human CD80 | 2D10 | FC |
PE/Cyanine5 anti-human CD80 | 2D10 | FC |
Purified anti-human CD80 | 2D10 | FC,WB,IP,IHC-F |
Alexa Fluor® 488 anti-human CD80 | 2D10 | FC |
Alexa Fluor® 647 anti-human CD80 | 2D10 | FC |
PE/Cyanine7 anti-human CD80 | 2D10 | FC |
APC anti-human CD80 | 2D10 | FC |
Brilliant Violet 650™ anti-human CD80 | 2D10 | FC |
Brilliant Violet 421™ anti-human CD80 | 2D10 | FC |
Brilliant Violet 605™ anti-human CD80 | 2D10 | FC |
PE/Dazzle™ 594 anti-human CD80 | 2D10 | FC |
PerCP/Cyanine5.5 anti-human CD80 | 2D10 | FC |
Brilliant Violet 510™ anti-human CD80 | 2D10 | FC |
Brilliant Violet 711™ anti-human CD80 | 2D10 | FC |
Brilliant Violet 785™ anti-human CD80 | 2D10 | FC |
TotalSeq™-A0005 anti-human CD80 | 2D10 | PG |
TotalSeq™-B0005 anti-human CD80 | 2D10 | PG |
TotalSeq™-C0005 anti-human CD80 | 2D10 | PG |
Ultra-LEAF™ Purified anti-human CD80 | 2D10 | FC,IP,WB,IHC-F |
Brilliant Violet 750™ anti-human CD80 | 2D10 | FC |
TotalSeq™-D0005 anti-human CD80 | 2D10 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained w... -
FITC anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with... -
PE anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with... -
PE/Cyanine5 anti-human CD80
-
Purified anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained w... -
Alexa Fluor® 488 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with... -
Alexa Fluor® 647 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Daudi stained with... -
PE/Cyanine7 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji stained ... -
APC anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stain... -
Brilliant Violet 650™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line, Raji was stained ... -
Brilliant Violet 421™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
Brilliant Violet 605™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
PE/Dazzle™ 594 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
PerCP/Cyanine5.5 anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stai... -
Brilliant Violet 510™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line, Raji was stained ... -
Brilliant Violet 711™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
Brilliant Violet 785™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
TotalSeq™-A0005 anti-human CD80
-
TotalSeq™-B0005 anti-human CD80
-
TotalSeq™-C0005 anti-human CD80
-
Ultra-LEAF™ Purified anti-human CD80
Daudi (human B Burkitt's lymphoma cell line) cells stained w... -
Brilliant Violet 750™ anti-human CD80
Human B-cell Burkitt's lymphoma cell line Raji was stained w... -
TotalSeq™-D0005 anti-human CD80
Follow Us