- Clone
- W20256A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell surface protein tactile, TACTILE, Cell surface antigen CD96, T cell-activated increased late expression protein
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
618753 | 100 µg | 287€ | ||||
618754 | 1 mg | 964€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD96, also known as TACTILE (T cell-activated increased late expression), is a type I transmembrane glycoprotein, belonging to the Ig superfamily. It is expressed on NK cells and T cells (upregulated after activation), but not on normal B cells and myeloid cells. CD96 is also found on some acute myeloid leukemia (AML) cells, and T cell acute lymphoblastic leukemia (T-ALL), as a leukemia stem cell-specific marker. A soluble form of CD96 has been found in serum. Human CD96, but not mouse CD96, is expressed as two isoforms due to alternative splicing. The main ligand of CD96 is CD155 (PVR), along with CD226 (DNAM-1) and TIGIT, and the binding is species-specific. Mouse CD96 has been reported to interact with nectin-1 (CD111), but human CD96 does not. CD96 may play an inhibitory role in immune responses. In CD96 deficient mice, NK cells produce more IFN-γ upon LPS treatment. In addition, CD96 negatively regulates NK cell function in mice, and blockade of CD96 with antibodies promotes anti-tumor responses and anti-metastatic activity in combination with anti-CTLA-4 or anti-PD-1. CD96 is considered to mediate activated T cell and NK cell adhesion to CD155-expressing target cells. CD96 also counterbalances CD226, which activates NK cell-mediated cytotoxicity, via competing CD155 binding.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD96 (TACTILE)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.5 µg/mL recombinant mouse CD96 (TACTILE) (Cat. No. 788804) to immobilized 2 µg/mL recombinant mouse CD155-Fc Chimera (Cat. No. 786606). ND50 = 0.1 - 1 µg/mL.
- RRID
-
AB_3068077 (BioLegend Cat. No. 618753)
AB_3068077 (BioLegend Cat. No. 618754)
Antigen Details
- Structure
- Ig superfamily
- Distribution
-
Positive on most peripheral T cells, NK cells and upregulated when activated
- Function
- CD96 is involved in NK cell adhesion and cytotoxicity via CD155
- Ligand/Receptor
- CD155, nectin 1
- Cell Type
- NK cells, NKT cells, T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- Soluble Receptors
- Antigen References
-
- Wang PL, et al. 1992. J Immunol. 148:2600-8.
- Meyer D, et al. 2009. J Biol Chem. 284:2235-44.
- Hosen N, et al. 2007. Proc Natl Acad Sci USA. 104:11008-13.
- Wen D, et al. 2015. Mol Clin Oncol. 3:833-838.
- Blake SJ, et al. 2016. Clin Cancer Res. 22:5183-5188.
- Chan CJ, et al. 2014. Nat Immunol. 15:431-8.
- Sanchez-Correa B, et al. 2019. Cancer. 11:877.
- Xu Z, Jin B. 2010. Cell Mol Immunol. 7:11-9.
- Georgiev H, et al. 2018. Front Immunol. 9:1072.
- Fuchs A, et al. 2004. J Immunol. 172:3994-8.
- Seth S, et al. 2007. Biochem Biophys Res Commun. 364:959-65.
- Gene ID
- 84544 View all products for this Gene ID
- UniProt
- View information about CD96 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD96 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-mouse CD96 (TACTILE) | W20256A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD96 (TACTILE)
Recombinant mouse CD96 (TACTILE) (Cat. No. 788804) (black ci...
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