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Ultra-LEAF™ Purified Human IgG4 Isotype Ctrl Recombinant Antibody

Pricing & Availability
Clone
QA16A15 (See other available formats)
Regulatory Status
RUO
Other Names
IGHG4, immunoglobulin heavy chain constant region gamma 4
Isotype
Human IgG4, κ
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
403701 100 µg 199€
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403702 1 mg 396€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Immunoglobulin G4, or IgG4, is a type of antibody. Humans express four types of IgG (IgG1, IgG2, IgG3, and IgG4). Antibodies in mammals are complex proteins, composed of four independent amino acid chains. The molecule is formed by two identical heavy chains and two identical light chains, and produced by B cells. The FcγR molecules (CD16, CD32, and CD64) on the surface of various immune cells can bind IgG1, IgG2, and IgG4.  

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Recombinant
Host Species
Human
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Elisa Control - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

QA16A15 is a recombinant human IgG4. It can be used as an isotype control when evaluating fully human, humanized, or chimeric antibodies. This product is suitable as an isotype-matched control for ELISA, immunoblotting, FACS, IHC, and functional studies.

Product Citations
  1. Balasko AL, et al. 2023. Sci Rep. 13:10082. PubMed
  2. Lee H, et al. 2023. Tuberc Respir Dis (Seoul). . PubMed
  3. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  4. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  5. Mandel I, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Sasaki T, et al. 2020. Int Immunol. 163:32. PubMed
  7. Assi HH, et al. 2021. Cancer Immunol Res. 9:1451. PubMed
  8. Takele Y, et al. 2022. PLoS Negl Trop Dis. 16:e0010544. PubMed
  9. Tahir S, et al. 2021. MAbs. 13:1961349. PubMed
  10. Phakham T, et al. 2021. Front Plant Sci. 12:736299. PubMed
  11. Fassler M, et al. 2021. J Neuroinflammation. 18:19. PubMed
  12. Thakkar D, et al. 2022. J Immunother Cancer. 10:. PubMed
  13. Smalley M, et al. 2020. iScience. 23:101229. PubMed
RRID
AB_3097034 (BioLegend Cat. No. 403701)
AB_3097034 (BioLegend Cat. No. 403702)

Antigen Details

Structure
Immunoglobulin, 150 KDa
Cell Type
B cells
Biology Area
Immunology
Gene ID
NA

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 09/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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