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PerCP/Cyanine5.5 anti-human CD123 Antibody

Pricing & Availability
Clone
6H6 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6H6_PerCPCyanine55_CD123_Antibody_1_110624
Human peripheral blood lymphocytes stained with CD123 (clone 6H6) PerCP/Cyanine5.5 and CD45RO (clone UCHL1) FITC
  • 6H6_PerCPCyanine55_CD123_Antibody_1_110624
    Human peripheral blood lymphocytes stained with CD123 (clone 6H6) PerCP/Cyanine5.5 and CD45RO (clone UCHL1) FITC
Compare all formats See PerCP/Cyanine5.5 spectral data
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306015 25 tests 141€
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306016 100 tests 306€
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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3Rα transfected COS cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.

Application References
  1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
  2. Herling M, et al. 2003. Blood 101:5007. (IHC)
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
  5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations
  1. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  2. Ferrari B, et al. 2022. J Clin Invest. 132:. PubMed
  3. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  4. Ait Djebbara S, et al. 2023. Front Immunol. 14:1180900. PubMed
  5. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  6. Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
  7. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  8. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  9. Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
  10. Jankeel A, et al. 2020. J Virol. :94. PubMed
  11. Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed
  12. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  13. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  14. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  15. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  16. Manuel S, et al. 2013. AIDS Res Hum Retroviruses. 29:1273. PubMed
  17. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  18. Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed
RRID
AB_2264693 (BioLegend Cat. No. 306015)
AB_2264693 (BioLegend Cat. No. 306016)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 04/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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