TotalSeq™ Reagents
TotalSeq-A: Contain a poly (A) capture sequence that mimics a natural mRNA. Designed to work with platforms that utilize poly (dT) oligonucleotides as the mRNA capture method. Compatible with 10x Genomics 3' Universal Gene Expression assay, captured via the poly (dT) sequence of the Gel Bead primers. Use of TotalSeq-A reagents with this assay requires reagent and protocol modifications; 10x Genomics provides only limited support for TotalSeq-A. For additional information regarding the use of TotalSeq-A reagents, please refer to our protocols or contact BioLegend Technical Support.
TotalSeq-B: The capture sequence is compatible with the Capture Sequence 1, or Partial Capture Sequence 1 of the barcoded Gel Bead primers used with the 10x Genomics 3' Universal Gene Expression or Flex Gene Expression assays, respectively. Use of TotalSeq-B antibodies with these assays is fully supported by 10x Genomics.
TotalSeq-C: The capture sequence is compatible with the Template Switch Oligo Capture Sequence, or the Partial Capture Sequence 1 of the barcoded Gel Bead primers used with the 10x Genomics 5' Universal Gene Expression or Flex Gene Expression assays, respectively. The use of TotalSeq-C antibodies with these assays is fully supported by 10x Genomics.
We offer single TotalSeq antibody conjugates, pre-titrated optimized cocktails, and hashtags for sample multiplexing in both human and mouse reactivity.
Explore BioLegend and 10x Genomics Data Sets
BioLegend TotalSeq Universal Cocktail Data Sets
10x Single Cell Gene Expression with Cell Surface Protein Labeling Data Sets
10x Single Cell Immune Profiling with Cell Surface Protein Data Sets
10x Fixed RNA Profiling with Cell Surface Protein Labeling Data Sets
Additional TotalSeq™ Resources
TotalSeq Resources and Learning
Barcode Look-Up - Access our barcode look-up table to view and export barcode information for all of our TotalSeq reagents
Follow Us