TotalSeq™ Reagents
TotalSeq-A: Designed to work with platforms that rely on poly(dT) oligonucleotides as the mRNA capture method. TotalSeq-A antibodies contain a poly(A) sequence that mimics a natural mRNA. Compatible with 10x Genomics Chromium Single Cell Expression Solution with Cell Surface Protein Labeling. Use of TotalSeq-A reagents with the 10x Single Cell Gene Expression requires reagent and protocol modifications; 10x Genomics provides only limited support for TotalSeq-A. For additional information regarding the use of TotalSeq-A reagents, please contact BioLegend Technical Support.
TotalSeq-B: Capture sequence is compatible with capture sequence 1 found on the Gel Beads used with the 10x Genomics Chromium Single Cell Gene Expression solution and 10x Genomics Single Cell Gene Expression Flex solution with Cell Surface Protein Labeling. Use of TotalSeq B with 10x his is fully supported by 10x Genomics.
TotalSeq-C: Capture sequence is compatible with the TSO capture sequence found on Single Cell 5' Gel Beads used with the 10x Genomics Chromium Single Cell Immune Profiling solution (5') which allows for immune repertoire profiling of T and B cell receptors at a single-cell resolution. TotalSeq-C is also compatible with the 10x Genomics Single Cell Gene Expression Flex solution. The use of TotalSeq-C antibodies is fully supported by 10x Genomics.
We offer single TotalSeq antibody conjugates, pre-titrated optimized cocktails, and hashtags for sample multiplexing in both human and mouse reactivity.
Explore BioLegend and 10x Genomics Data Sets
BioLegend TotalSeq Universal Cocktail Data Sets
10x Single Cell Gene Expression with Cell Surface Protein Labeling Data Sets
10x Single Cell Immune Profiling with Cell Surface Protein Data Sets
10x Fixed RNA Profiling with Cell Surface Protein Labeling Data Sets
Additional TotalSeq™ Resources
TotalSeq Resources and Learning
Barcode Look-Up - Access our barcode look-up table to view and export barcode information for all of our TotalSeq reagents
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