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5 Reasons to Use Veri-Cells™
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| Reliable, consistent experimental controls are paramount for validating reagent performance in biological research. Our Veri-Cells™ are lyophilized cells designed to be used for flow cytometry antibody validation. While they're useful as controls in multi-center or longitudinal studies to monitor assay consistency, Veri-Cells™ would be useful for anyone doing flow cytometry research! In this blog, we'll outline 5 reasons why you should use Veri-Cells™, and what makes them the right product for you! |
1) Stability |
| Our Veri-Cells™ have excellent long-term stability - two years in its lyophilized form, and five days post-reconstitution. Our Veri-Cells™ are available in smaller sizes so you don't need to reconstitute larger batches at once and can keep them lyophilized for long-term storage and use in the future. |
2) Validity |
| Veri-Cells™ PBMC and Veri-Cells™ Leukocytes have been validated for expression of over 150 surface markers using our LEGENDScreen™ Human PE Kit. Additionally, we have validated intracellular expression of commonly examined markers Granzyme B, Perforin, FOXP3, and Helios. You can check out the listing of validated markers, as well as additional stability information and representative data, on the Veri-Cells™ section of our flow cytometry control page. |
3) Accessibility, convenience |
| Veri-Cells™ are perfect for those who may have difficulty accessing human blood, or do not have the time to enrich blood cells and freeze them. We deliver our products overnight within US and Canada (and some European destinations from our UK location). Once you order your Veri-Cells™, they can arrive in your lab as early as the next morning. You can purchase them in bulk, hold onto the lyophilized product, and just reconstitute a vial of cells when necessary. |
4) Variety |
| We offer an ever-expanding variety of Veri-Cells™ products, depending on the target antigens you want to observe. This allows you to forego setting up cumbersome stimulation experiments you may not have performed on your own, and obtain control samples containing markers/cells that are otherwise difficult to detect in normal blood, such as CD34+ hematopoietic stem cells. |
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| Examples of Veri-Cells™ products designed to serve as controls to detect markers that normally require stimulation (Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway), left plots) and are difficult to detect in normal human blood (Veri-Cells™ CD34 PBMC, right plots). Check out their respective webpages to learn more about these products! |
| Don't see cells and/or desired pre-treatment conditions youre looking for? We're open to taking on custom projects to generate a lyophilized cell control to fit your needs. If you're interested, contact us at tech@biolegend.com for more details. |
5) Consistency, reliability |
| We analyze every lot of Veri-Cells™ produced for a select number of phenotyping markers to ensure that the cell populations/marker staining is within the expected frequency. Observed experimental values from our QC testing are listed on their certificates of analyses, so you have a point of reference for the vial of cells you have on-hand. Of course, this is subject to some degree of variability (e.g. clone of antibody being used to stain, gating for analysis, etc.), but you'll have peace of mind knowing that the vial you received has been tested in comparison to a gold standard to ensure relative consistency. As with all of our reagents, our Veri-Cells™ are also backed by our 100% satisfaction guarantee. |
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| Two Veri-Cells™ PBMC lots reconstituted and stained on different days, and ran on separate flow cytometers. Gating is based on unstained Veri-Cells™ PBMC controls ran simultaneously for each run. The CD4 vs. CD8 plot is on CD3+CD19- gated cells, and the histogram for CD56, CD16 is plotted on CD3-CD19- cells. |
| Contributed by Kenta Yamamoto, PhD. |
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