Prior to performing bioassay validation and stability testing, we test each recombinant protein for product purity and quantification. In the table below you can find examples of the attributes tested, the test performed, and some acceptance criteria used to ensure that our customers are confident they are receiving the highest quality reagents.

Parameter Testing

Purity

SDS-PAGE, FPLC, (>95%)

Quantity

UV Spec, SDS-PAGE

Molecular Weight

DTT-reduced and non-reduced SDS-PAGE

Microbiological Control

0.2 μm Filtration

Endotoxin Level

LAL Assay (<0.1 ng/μg protein or <1 EU/μg protein)

Recombinant proteins were run in SDS-PAGE under either DTT-reducing or non-reducing conditions, as indicated, to confirm purity of the recombinant protein was >95%.

Recombinant proteins run on an LC-Agilent 6230 Time-of-Flight Mass Spectrometer. Deconvoluted mass spectrums shown with predicted and observed molecular weights indicated.

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