Cell Proliferation

Tracking cell proliferation

 

Proliferation of cells can be measured using probes that are retained by daughter cells, nuclear markers that are only expressed by cycling cells, or assays that assess metabolic activity. The table below summarizes kits and reagents for tracking cell proliferation.

 

Reagent or Kit	Description
CFSE	Retained in daughter cells following cell division, can be used for ex vivo and in vivo cell proliferation assays. Its peak excitation and emission wavelengths are 492 nm and 517 nm, respectively.
Tag-it™ Violet	Retained in daughter cells following cell division, can be used for ex vivo and in vivo cell proliferation assays. Its peak excitation and emission wavelengths are 395 nm and 455 nm, respectively.
BrdU	Incorporated into newly synthesized DNA, and can be detected with anti-BrdU antibodies.
Phase-Flow™ BrdU kits	Complete set of reagents for analyzing BrdU incorporation by flow cytometry. Kit includes BrdU pulsing solution, anti-BrdU antibody, all necessary buffers, and DNA dyes DAPI and 7-AAD.
Ki-67	Nuclear protein expressed only in cycling cells, and not found in quiescent or senescent (G0) cells.
Deep Blue Cell Viability™	Measures resazurin reduction to determine cell metabolic activity and rate.
LDH-Cytox Assay™ Kit	Measures tetrazolium salt reduction resulting from lactate dehydrogenase (LDH) activity. Since LDH is released from damaged cells, this assay can be used to determine cytotoxicity.