Flow Cytometry Controls

Veri-Cells™...

Are most appropriate when you need reference/control cells for longitudinal, multi-center studies.

Products

Reference laboratories, clinical research organizations, and multi-center clinical trials, among other institutions, need reliable controls to monitor assay performance and variability for longitudinal studies. Veri-Cells™ is our line of lyophilized control cell products, which can be used to help monitor data quality and aid in reproducibility in multi-center and longitudinal studies. 

Veri-Cells™ feature:

• Exceptional long term stability
• Scatter profile similar to fresh samples
• Validation for the detection of over 150 markers

For more helpful information on Veri-Cells™, visit the FAQ page.

Sections:

Veri-Cells™ PBMC (CD103, CD117)

Veri-Cells™ CD138 Leukocytes

Veri-Cells™ Heavy Metal (Ta) PBMC

Veri-Cells™ Activated (Cytokine) PBMC

Veri-Cells™ Activated (Surface)

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway)

Veri-Cells™ CD34 PBMC

Veri-Cells™ Leukocytes

Veri-Cells™ CD4-Low PBMC

Veri-Cells™ PBMC

Veri-Cells™ PBMC (CD103, CD117)

Veri-Cells™ PBMC (CD103, CD117) Kit can be used as a staining control that provides assay values for CD103, CD1a, CD117, CD30, and CD71 expression. For best results, Cell Staining Buffer (Cat. No. 420201) is recommended for all washing steps. Please note that any claims relative to product performance are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.

Veri-Cells™ PBMC (CD103, CD117) were stained with:

• Fig. A: anti-human CD14 PE/Cyanine5 and anti-human CD19 FITC (right). Cells were gated on forward and side scatter (left).
• Fig. B: anti-human CD14 PE/Cyanine5, anti-human CD19 FITC, and anti-human CD103 PE/Cyanine7 (left) or Mouse IgG1, κ PE/Cyanine7 isotype control (right). CD14- CD19- double negative single cells are shown.
• Fig. C: anti-human CD14 PE/Cyanine5, anti-human CD19 FITC, and anti-human CD1a BV711™ (left) or Mouse IgG1, κ BV711™ isotype control (right). CD14- CD19- double negative single cells are shown.
• Fig. D: anti-human CD14 PE/Cyanine5, anti-human CD19 FITC, and anti-human CD117 PE (left) or Mouse IgG1, κ PE isotype control (right). CD14- CD19- double negative single cells are shown. 
• Fig. E: anti-human CD14 PE/Cyanine5, anti-human CD19 (clone HIB19) FITC, and anti-human CD30 (clone BY88) APC (left) or Mouse IgG1, κ APC isotype control (right). CD14- CD19- double negative single cells are shown.
• Fig F: anti-human CD14 PE/Cyanine5, anti-human CD19 FITC, and anti-human CD71 BV650™ (left) or Mouse IgG2a, κ BV650™ (right). CD14- CD19- double negative single cells are shown.

Veri-Cells™ CD138 Leukocytes

Veri-Cells™ CD138 Leukocytes contain a mixture of human CD138+ cells and other leukocytes and can be used as controls to verify the expression of CD138, CD38, CD56 and CD269. In addition, Veri-Cells™ CD138 Leukocytes have been verified for expression of common markers, including CD3, CD4, CD8, CD19, CD20, CD56/16, CD14, CD25, CD69, HLA-DR, Granzyme B, Perforin, FOXP3, and Helios.

Veri-Cells™ CD138 Leukocytes were stained with anti-human CD45 (clone 2D1) PE, anti-human CD19 (clone SJ25C1) APC, anti-human CD38 (clone HIT2) FITC, and anti-human CD138 (clone MI15) Brilliant Violet 421™. CD45- and CD19- negative cells (left) were analyzed for CD138 and CD38 expression (right). Gates for the CD38 vs CD138 plot were set based on corresponding isotype controls.

Veri-Cells™ CD138 Leukocytes were stained with Human TBNK 6 Color Cocktail (Cat. No. 391503). CD45+ CD3+ and CD45+ CD3- cells (top right) were gated on lymphocytes (top left) and analyzed for lineage marker expression (bottom left/right).

Veri-Cells™ Heavy Metal (Ta) PBMC

Veri-Cells™ Heavy Metal (Ta) PBMCs have been labeled with the heavy metal tantalum. When mixed with sample cells, they can be separated from the cells of interest using the tantalum signal. They have been verified to stain for commonly tested surface markers including CD3, CD4, CD8, CD14, CD16, CD19, CD38, CD123, and HLA-DR.

Veri-Cells™ Heavy Metal (Ta) PBMCs were mixed with whole blood from different donors. Mixed samples were stained with a heavy-metal labeled anti-CD14 antibody and run on a CyTOF®. Median intensity of CD14 signal was measured for the blood sample alone (top row) and normalized to the median intensity of CD14 signal on Veri-Cells™ in each sample (bottom row). Normalized median values are indicated. Data provided by Adeeb Rahman, PhD, Icahn School of Medicine at Mt. Sinai.

Veri-Cells™ Activated (Cytokine) PBMC

Veri-Cells™ Activated (Cytokine) PBMC have been activated with our Cell Activation Cocktail (with Brefeldin A), which includes phorbol 12-myristate 13-acetate (PMA) and Ionomycin, for 6 hours. They have been validated to stain for activation-induced cytokines including IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17, GM-CSF, IFN-γ, and TNF-α, along with surface markers typically expressed on human PBMCs. Cells do not require additional fixation nor permeabilization prior to cytokine staining, thus saving more time in your experiment.

Veri-Cells™ Activated (Cytokine) PBMCs were stained with anti-CD3 FITC and either anti-TNF-α PerCP/Cyanine5.5, anti-IL-17A BV421™, anti-GM-CSF Pacific Blue™, or anti-IFN-γ APC. Plots were gated based on staining with the corresponding isotype controls as indicated.

Veri-Cells™ Activated (Surface)

Veri-Cells™ Activated (Surface) have been activated with Concanavalin A for 72 hours. This makes them ideal for the detection of T cell activation markers, including CD25, CD69, CD134, CD279, CD366, and TIGIT.

Veri-Cells™ Activated (Surface) PBMCs were stained with anti-CD4 FITC and either anti-CD25 PerCP/Cyanine5.5, anti-CD134 APC, anti-CD69 APC, or anti-TIGIT APC. Plots were generated on CD3⁺ cells and gated based on staining with the corresponding isotype control as indicated.

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway)

Veri-Cells™ Phospho PBMC are stimulated with PMA/Ionomycin for 15 minutes. This product includes both stimulated and vehicle control cells for the staining of RAS mitogen-activated kinase pathways, including phospho-S6, phospho-p38, and phospho-ERK1/2.

Veri-Cells™ Phospho PBMC (MAPK/ERK Pathway) vehicle control (open histogram) or stimulated cells (closed histogram) were stained with anti-phospho S6-Alexa Fluor® 488 (left), anti-phospho p38-PE (center) or anti-human phospho ERK-Alexa Fluor® 647 (right). The histograms are gated on the lymphocyte population.

Veri-Cells™ CD34 PBMC

Veri-Cells™ CD34 PBMC contains mixture of CD34+ KG-1a cells and human PBMCs to help verify CD34 antibody binding without the need for artificial enrichment or the collection of a large number of events.

Veri-Cells™ CD34 PBMCs were stained with anti-CD45 (clone HI30) FITC and anti-CD34 (clone 561) PE. Dot plots have excluded doublets from analysis.

Veri-Cells™ Leukocytes 

Unlike Veri-Cells™ PBMC and CD4-Low PBMC, Veri-Cells™ Leukocytes contain neutrophils and eosinophil populations. These cells can be used as controls or reference material to monitor expression of granulocyte markers such as CD15 and CD16.

Veri-Cells™ Leukocytes were surface stained with anti-CD8 Alexa Fluor® 700, then fixed and permeabilized before staining with anti-Granzyme B FITC, and anti-Perforin PE. Scatter profile of the cells is shown on the left.

Veri-Cells™ CD4-Low PBMC

Veri-Cells™ CD4-Low PBMC are specially formulated to contain a lower frequency of CD4+ cells, similar to that observed in patients with CD4 immunodeficiency. The expected frequency of CD3+ CD4+ cells can be found on the certificate of analysis for that lot.

Veri-Cells™ PBMCs and CD4-low PBMCs were stained with anti-CD4 PE/Cyanine7 and anti-CD3 FITC and the frequency of CD4⁺ cells is indicated. Plots were first gated on the lymphocyte population.

Veri-Cells™ PBMC

Veri-Cells™ PBMC consist of lyophilized human PBMCs validated for over 150 surface markers and intracellular targets and exhibit a scatter profile similar to freshly isolated cells. Please refer to the Validation Tab to view the full listing of markers and clones that can be used with Veri-Cells™ PBMC.