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Video Guide: Flow Cytometry

Flow cytometry is a flexible application that allows researchers to study surface, intracellular, and nuclear protein expression. Static written protocols can be difficult to follow or interpret. In those situations, it can help to have full videos that guide you through each step or webinars that explain the utility of a reagent.

Fill out our form to gain access to the following helpful videos:

  • Isolating PBMCs with Lymphopure™: Watch to learn how you can reliably isolate mononuclear cells from whole blood in just 5 easy steps using Lymphopure™ density gradient medium.
     
  • Surface and Intracellular Cytokine Staining for Flow Cytometry: This video provides a list of the reagents required and the steps needed to perform flow cytometry staining, covering fixation, permeabilization, and both surface and intracellular staining with antibodies.
     
  • True-Nuclear™ Buffer Set Webinar: In this mini-webinar, scientist David Soper, discusses the advantages of BioLegend’s True-Nuclear™ Transcription Factor Buffer Set over the FOXP3 Buffer Set.
     
  • Intracellular Phospho Staining for Flow Cytometry: Protein phosphorylation is a critical step in a wide variety of signaling pathways. Watch this video to learn how to use True-Phos™ Perm Buffer to detect these phosphorylated targets by flow cytometry.

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