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Organic Polymers

Brilliant Violet™ dyes are organic, light-emitting, diode polymers whose brightness is related to the length of the polymer, but whose spectra remain stable no matter the polymer’s length. These dyes have a high extinction coefficient and quantum yield resulting in high intensity brightness. Brilliant Violet™ dyes are effective over a wide pH range and are compatible with a variety of fixatives.

Brilliant Violet 421™


Brightness: 5
Ex Laser: 405 nm
Ex Max: 405 nm
Em Max: 421 nm
Filter Used: 450/50
Molecular Weight: 60-80 kDa
Recommended Application(s):
Flow Cytometry, Microscopy

Fluorophore at a Glance:

  • A very potent donor with an extinction coefficient of 2,500,000 M-1cm-1 which makes for very potent tandem dyes.
  • In some cases, it can exhibit non-specific binding to itself.
  • Very bright, ideal for detecting low-expressed markers.
 

View all Brilliant Violet 421™ Products.
 

Learn more about Brilliant Violet Dyes.

BV421™ withstands a variety of fixatives

Improved Signal to Noise as compared to Pacific Blue™


Human peripheral blood lymphocytes stained with anti-CD127conjugated to BV421™ and Pacific Blue™ at varying dilutions.

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