- Clone
- 1B4/CD18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin beta-2, integrin beta chain, beta 2
- Isotype
- Mouse IgG2a, κ
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Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 373403 | 25 tests | $147.00 | |||
| 373404 | 100 tests | $323.00 | |||
CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human peripheral blood lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes.
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Application References
(PubMed link indicates BioLegend citation) -
- Singer II, et al. 1993. J. Immunol. 150:2844.
- Seth R, et al. 1991. FEBS Lett. 282:193.
- RRID
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AB_2716019 (BioLegend Cat. No. 373403)
AB_2716019 (BioLegend Cat. No. 373404)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein
- Distribution
-
Leukocytes
- Function
- Adhesion and signaling in hematopoietic cells
- Interaction
- Associates with CD11a, CD11b, or CD11c
- Ligand/Receptor
- ICAMs
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Hogg N, et al. 1999. J. Clin. Invest. 103:97.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Springer TA. 1994. Cell 76:301. - Gene ID
- 3689 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
Other Formats
View All CD18 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD18 | 1B4/CD18 | FC |
| APC anti-human CD18 | 1B4/CD18 | FC |
| PE/Cyanine7 anti-human CD18 | 1B4/CD18 | FC |
| Alexa Fluor® 488 anti-human CD18 | 1B4/CD18 | FC |
| PE anti-human CD18 | 1B4/CD18 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD18
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PE/Cyanine7 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
Alexa Fluor® 488 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PE anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c...