Alexa Fluor® 488 anti-human Cytokeratin 7 Antibody

Pricing & Availability
Clone
W16155A (See other available formats)
Regulatory Status
RUO
Other Names
Keratin type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Type-II keratin Kb7, Sarcolectin, SCL, Cytokeratin 7, Keratin 7
Isotype
Rat IgG2a, κ
1_W16155A_A488_Cytokeratin-7_ICC_031318
HaCaT cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 488 rat IgG2a (Cat. No. 400525) or with Alexa Fluor® 488 anti-human Cytokeratin 7 antibody (green) at (B) 1:100 (5 µg/ml) and (C) 1:200 (2.5 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 1_W16155A_A488_Cytokeratin-7_ICC_031318
    HaCaT cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 488 rat IgG2a (Cat. No. 400525) or with Alexa Fluor® 488 anti-human Cytokeratin 7 antibody (green) at (B) 1:100 (5 µg/ml) and (C) 1:200 (2.5 µg/ml) dilution. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 2_19_Human_Liver_Keratin7_CD44_CD45
    Confocal image of human liver sample acquired using the IBEX method of highly multiplexed antibody-based imaging: Cytokeratin 7 (green) in Cycle 1, CD44 (blue) in Cycle 4, and CD45 (magenta) in Cycle 4. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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601605 25 µg $159.00
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601606 100 µg $370.00
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Description

Keratin 7 (KRT7) belongs to type II keratin and is expressed in the epithelia lining the cavities of internal organs, gland ducts and blood vessels. The function of KRT7 is still not clear but it has been suggested involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). In cancer, the expression of KRT7 is observed in many types of carcinoma and is associated with neoplasm progression. The diverse and unique expression pattern of KRT7 is often used as a diagnosis indicator in epithelial origin carcinoma. For example, aberrant KRT7 expression is observed in triple negative breast cancer. In bladder cancer, loss of KRT7 results in promoting proliferation of the bladder urothelium.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human KRT7 peptide (446-462) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml (1:100-1:200 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This antibody does not react with mouse (in-house tested). Additional reported applications (for the relevant formats) include: spatial biology (IBEX)1,2.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2728458 (BioLegend Cat. No. 601605)
AB_2728458 (BioLegend Cat. No. 601606)

Antigen Details

Structure
Type II cytokeratin. 469 amino acids with a predicted molecular weight of 51 kD.
Distribution

Cytoplasm.

Function
KRT7 is a type II keratin, involved in the translational regulation of the human papillomavirus type 16 E7 mRNA.
Interaction
KRT7 interacts with EIF3S10, HPV16 E7, and GPER1.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Tajima Y, et al. 2017. Sci Rep. 7:40684.
2. Huang B, et al. 2016. Oncogene. 35:4927.
3. Szponar A, et al. 2012. Virchows Arch. 460:423.
4. Sano M, et al. 2010. Int J Oncol. 36:321.

Gene ID
3855 View all products for this Gene ID
UniProt
View information about Cytokeratin 7 on UniProt.org
Go To Top Version: 2    Revision Date: 04/26/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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