- Clone
- I21/7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL,
- Isotype
- Rat IgG2a, κ
-
C57BL/6 mouse splenocytes were stained with CD11a (clone I21/7) Alexa Fluor® 488 (filled histogram) or rat IgG2a, κ isotype control Alexa Fluor® 488 (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 153105 | 25 µg | $101.00 | |||
| 153106 | 100 µg | $223.00 | |||
CD11a is a 180 kD glycoprotein, also known as αL integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Whole T cells Lymphoma BW5147 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Trowbridge IS, et al. 1981. J. Exp. Med. 154:1517.
- Champe M, et al. 1995. J. Biochem. 270:1388.
- Product Citations
-
- RRID
-
AB_2716035 (BioLegend Cat. No. 153105)
AB_2716035 (BioLegend Cat. No. 153106)
Antigen Details
- Structure
- Integrin, associated with integrin CD18, 180 kD
- Distribution
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Lymphocytes, granulocytes, monocytes, macrophages
- Function
- Intercellular adhesion
- Interaction
- Associates with CD18
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2(CD102), ICAM-3(CD50)
- Cell Type
- Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479. - Gene ID
- 16408 View all products for this Gene ID
- UniProt
- View information about CD11a on UniProt.org
Other Formats
View All CD11a Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD11a | I21/7 | FC |
| Alexa Fluor® 488 anti-mouse CD11a | I21/7 | FC |
| PE anti-mouse CD11a | I21/7 | FC |
| PE/Cyanine7 anti-mouse CD11a | I21/7 | FC |
| APC anti-mouse CD11a | I21/7 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD11a
C57BL/6 mouse splenocytes were stained with purified CD11a (... -
Alexa Fluor® 488 anti-mouse CD11a
C57BL/6 mouse splenocytes were stained with CD11a (clone I21... -
PE anti-mouse CD11a
C57BL/6 mouse splenocytes were stained with CD11a (clone I21... -
PE/Cyanine7 anti-mouse CD11a
C57BL/6 mouse splenocytes were stained with CD11a (clone I21... -
APC anti-mouse CD11a
C57BL/6 mouse splenocytes were stained with CD11a (clone I21...