Alexa Fluor® 488 anti-mouse IL-17A Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli expressed, recombinant mouse IL-17A

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser

Blue Laser (488 nm)

Application Notes

ELISA Capture^3,4 and ELISPOT Capture⁵: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry^{2-4,7,8,11,12}: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization^6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)

1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed

Product Citations

1. Kim E, et al. 2022. Immunity. 55:145. PubMed
2. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
3. Gültner S, et al. 2010. Eur J Immunol. 40:3403. PubMed
4. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
5. Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
6. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
7. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
8. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
9. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
10. Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed
11. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
12. Obregón-Henao A, et al. 2013. PLoS One. 8:80669. PubMed
13. Liu S, et al. 2017. J Pharmacol Sci. 10.1016/j.jphs.2017.02.001. PubMed
14. Maston LD, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L609. PubMed
15. Kim E, et al. 2022. STAR Protoc. 3:101366. PubMed
16. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
17. Lu M, et al. 2021. J Virol. 95:e0059221. PubMed
18. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
19. Dudeck J, et al. 2019. J Allergy Clin Immunol. 143:1849. PubMed
20. Cheng HW, et al. 2022. Nat Commun. 13:2027. PubMed
21. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
22. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
23. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed

RRID

AB_536011 (BioLegend Cat. No. 506909)
AB_536011 (BioLegend Cat. No. 506910)

Antigen Details

Structure

Cytokine; dimer; 15 kD (Mammalian).

Bioactivity

Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells

Cell Sources

CD4⁺ memory T cells

Cell Targets

Fibroblasts, epithelial and endothelial cells, stromal cells

Receptors

IL-17R (CD217)

Biology Area

Cell Biology, Immunology, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID

16171 View all products for this Gene ID

UniProt

View information about IL-17A on UniProt.org

Related Protocols

• Surface and Intracellular Cytokine Staining for Flow Cytometry - Video
• Intracellular Flow Cytometry Staining Protocol

Other Formats