- Clone
- S16009B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prf1, Pfp, Pfn, Pfn-1
- Isotype
- Rat IgG2a, κ
-
C57BL/6 splenocytes were surface stained with anti-mouse CD49b (clone DX5) PE. Cells were then fixed, permeabilized, and then intracellularly stained with anti-mouse Perforin (clone S16009B) Alexa Fluor® 488 (left) or rat IgG2a, κ Alexa Fluor® 488 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 154409 | 25 µg | $159.00 | |||
| 154410 | 100 µg | $370.00 | |||
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse perforin transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone S16009B reacts with perforin from both C57BL/6 and BALB/c strains.
- RRID
-
AB_3097554 (BioLegend Cat. No. 154409)
AB_3097554 (BioLegend Cat. No. 154410)
Antigen Details
- Structure
- 70 kD Protein that oligomerizes for pore formation.
- Distribution
-
CTL, NK (Cytoplasmic Granules)
- Function
- Target cell lysis
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Podack ER, et al, 1988, Immunol Rev, 103:203
- Konjar S, et al, 2010. Immunology, 131:257
- Trapani JA, et al, 2002, Nat Rev Immunol, 2:735
- Osinska I, et al. 2014, Cent Eur J Immnol, 39:109
- Gene ID
- 18646 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Other Formats
View All Perforin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse Perforin | S16009B | ICFC |
| APC anti-mouse Perforin | S16009B | ICFC |
| PE anti-mouse Perforin | S16009B | ICFC |
| Pacific Blue™ anti-mouse Perforin | S16009B | ICFC |
| Alexa Fluor® 488 anti-mouse Perforin | S16009B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Perforin
C57BL/6 splenocytes were fixed, permeabilized, and stained w... 
-
APC anti-mouse Perforin
C57BL/6 splenocytes were stained with CD49b. Cells were fixe... 
-
PE anti-mouse Perforin
C57BL/6 splenocytes were stained with CD49b. Cells were fix... 
-
Pacific Blue™ anti-mouse Perforin
C57BL/6 splenocytes were stained with anti-mouse CD49b PE, f... -
Alexa Fluor® 488 anti-mouse Perforin
C57BL/6 splenocytes were surface stained with anti-mouse CD4...