Alexa Fluor® 488 anti-rat CD45RA Antibody

Pricing & Availability
Clone
OX-33 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte common antigen, high molecular weight form
Isotype
Mouse IgG1, κ
OX-33_Alx488_021308
LOU rat splenocytes stained with OX-33 Alexa Fluor® 488
  • OX-33_Alx488_021308
    LOU rat splenocytes stained with OX-33 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
202310 100 µg $229.00
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Description

CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Leukocyte common antigen purified from rat splenocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Smith T, et al. 2000. Nature Med. 6:62. (IHC)
RRID
AB_572043 (BioLegend Cat. No. 202310)

Antigen Details

Structure
Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD
Distribution

Primarily on B lymphocytes

Function
B cell receptor signal transduction
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.

Gene ID
24699 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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