Alexa Fluor® 594 anti-Histone H3-Phosphorylated (Ser28) Antibody

Pricing & Availability
Clone
HTA28 (See other available formats)
Regulatory Status
RUO
Other Names
Histone-H3
Isotype
Rat IgG2a, κ
HTA28_A594_Histone_Antibody_020918
HeLa cells were were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with 1: 2500 diluted (0.2 µg/ml, A) Alexa Fluor® 594 Rat IgG2a, κ isotype control antibody or 1: 2500 diluted (0.2 µg/ml, B) and 1: 10000 diluted (0.05 µg/ml, C) Alexa Fluor® 594 anti-Histone H3-Phosphorylated (Ser28) antibody (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. The images displayed HeLa cells at prophase in cell cycle.
  • HTA28_A594_Histone_Antibody_020918
    HeLa cells were were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with 1: 2500 diluted (0.2 µg/ml, A) Alexa Fluor® 594 Rat IgG2a, κ isotype control antibody or 1: 2500 diluted (0.2 µg/ml, B) and 1: 10000 diluted (0.05 µg/ml, C) Alexa Fluor® 594 anti-Histone H3-Phosphorylated (Ser28) antibody (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. The images displayed HeLa cells at prophase in cell cycle.
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641015 25 µg $159.00
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641016 100 µg $370.00
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Description

H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of human histone H3.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of  0.05 - 0.2 μg/ml (1:2500-1:10000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Hirata A, et al. 2004. J. Histochem. Cytochem. 52:1503.
  2. Goto H, et al. 1999. J. Biol. Chem. 274:25543.
  3. Ozawa K. 2008. Cytometry A 73:517.
  4. Goode NJ, et al. 2014. PLoS Genet. 10:1004323. PubMed
RRID
AB_2728471 (BioLegend Cat. No. 641015)
AB_2728471 (BioLegend Cat. No. 641016)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.

Regulation
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID
8290 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 1    Revision Date: 02/14/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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