Pricing & Availability
Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 594 (red), 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (yellow), and 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Compare all formats
See Alexa Fluor® 594 spectral data
Technical data sheet
- Clone
- HIB19 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD19.11
- Other Names
- B4
- Isotype
- Mouse IgG1, κ
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Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml CD19 (clone HIB19) Alexa Fluor® 594 (red), 10 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (yellow), and 10 µg/ml CD14 (clone HCD14) Alexa Fluor® 647 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective. -
Human frozen tonsil tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 5 µg/mL of Alexa Fluor® 647 anti-human CD3 antibody (blue) and Alexa Fluor® 594 anti-human CD19 antibody (red) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 302250 | 100 µg | $293.00 | |||
Description
CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).
Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing
-
Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Bradbury L, et al. 1993. J. Immunol. 151:2915.
- Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
- Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
- Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- RRID
-
AB_2563233 (BioLegend Cat. No. 302250)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 95 kD
- Distribution
-
B lineage (except plasma cells), follicular dendritic cells
- Function
- B cell activation and differentiation
- Ligand/Receptor
- Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915. - Gene ID
- 930 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
Other Formats
View All CD19 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 APC -
Biotin anti-human CD19
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 FITC -
PE anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PE -
PE/Cyanine5 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PE/Cya... -
Purified anti-human CD19
Human peripheral blood lymphocytes stained with purified HIB... 
Human frozen spleen tissue slices were fixed with 4% PFA for... -
APC/Cyanine7 anti-human CD19
Human peripheral blood lymphocytes were stained with CD20 FI... -
PE/Cyanine7 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PE/Cya... -
Alexa Fluor® 488 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Alexa ... 
Human peripheral blood mononuclear cells were fixed with 2% ... -
Alexa Fluor® 647 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Alexa ... 
Human frozen tonsil tissue slices were fixed with 4% PFA for... -
Pacific Blue™ anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Pacifi... -
Alexa Fluor® 700 anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 Alexa ... -
PerCP anti-human CD19
Human peripheral blood lymphocytes stained with HIB19 PerCP -
PerCP/Cyanine5.5 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Brilliant Violet 421™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FIT... 
Human peripheral mononuclear cells were fixed with 2% parafo... 
Human frozen tonsil tissue slices were fixed with 4% PFA for... -
Brilliant Violet 570™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Brilliant Violet 650™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 785™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 510™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 605™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Brilliant Violet 711™ anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Purified anti-human CD19 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 142Nd-an... -
Alexa Fluor® 594 anti-human CD19
Human peripheral blood mononuclear cells were fixed with 2% ... 
Human frozen tonsil tissue slices were fixed with 4% PFA for... -
PE/Dazzle™ 594 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
PE anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD19
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Pacific Blue™ anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0050 anti-human CD19
-
Brilliant Violet 750™ anti-human CD19
Human peripheral blood lymphocytes stained with CD19 (clone ... -
TotalSeq™-B0050 anti-human CD19
-
TotalSeq™-C0050 anti-human CD19
-
PerCP/Cyanine5.5 anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
Spark NIR™ 685 anti-human CD19
Human peripheral blood lymphocytes were stained with CD19 (c... -
Ultra-LEAF™ Purified anti-human CD19
Human peripheral blood lymphocytes stained with Ultra-LEAF™ ... -
APC/Fire™ 810 anti-human CD19
Human peripheral blood lymphocytes were stained with CD20 FI... -
PE/Fire™ 640 anti-human CD19
Human peripheral blood lymphocytes were stained with CD20 FI... -
PE/Fire™ 700 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0050 anti-human CD19
-
Spark YG™ 593 anti-human CD19
Human peripheral blood lymphocytes were stained with CD20 Al... -
GMP Pacific Blue™ anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
KIRAVIA Blue 520™ anti-human CD19
Human peripheral blood lymphocytes stained with anti-human C... -
GMP PerCP/Cyanine5.5 anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 500 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Violet™ 423 anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 750 anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine5 anti-human CD19
Typical results from human peripheral blood lymphocytes stai... -
Alexa Fluor® 660 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 806 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV395™ anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD19
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD19
Typical results from human peripheral blood lymphocytes stai...