- Clone
- C20Mab-60 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B1, Bp35
- Isotype
- Mouse IgG2a, κ
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IHC staining of Alexa Fluor® 594 anti-human CD20 (clone C20Mab-60) on paraffin-embedded human spleen tissue. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 5 µg/mL of anti-human CD8a Alexa Fluor® 647 (green) and 10 µg/mL of anti-human CD20 (clone C20Mab-60) Alexa Fluor® 594 (red) (panel A) or mouse IgG2a, κ Alexa Fluor® 594 isotype control (red) (panel B) at 4°C overnight. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm -
Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and anti-human CD20 (clone C20Mab-60) Alexa Fluor® 594 (left), or mouse IgG2a, κ Alexa Fluor® 594 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 382805 | 25 µg | $175.00 | |||
| 382806 | 100 µg | $265.00 | |||
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- LN229/CD20 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
FC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - RRID
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AB_3068270 (BioLegend Cat. No. 382805)
AB_3068270 (BioLegend Cat. No. 382806)
Antigen Details
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
-
B cell, T cell subsets
- Function
- B cell activation
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Cell Type
- B cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Furusawa Y, et al. 2020. Monoclon Antib Immunodiagn Immunother. 39:112-116.
- Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Other Formats
View All CD20 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD20 | C20Mab-60 | FC,IHC-P,WB |
| Alexa Fluor® 647 anti-human CD20 | C20Mab-60 | FC,IHC-P |
| Alexa Fluor® 594 anti-human CD20 | C20Mab-60 | IHC-P,FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD20
Human peripheral blood lymphocytes were surface stained with... 
Human paraffin-embedded spleen tissue slices were prepared w... 
Human paraffin-embedded tonsil tissue slices were prepared w... Human paraffin-embedded B cell lymphoma tissue slices were p... -
Alexa Fluor® 647 anti-human CD20
Human peripheral blood lymphocytes were stained with anti-hu... 
IHC staining of anti-human CD20 (clone C20Mab-60) Alexa Fluo... -
Alexa Fluor® 594 anti-human CD20
IHC staining of Alexa Fluor® 594 anti-human CD20 (clone ... 
Human peripheral blood lymphocytes were stained with anti-hu...
