- Clone
- 11A1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- C4.4A, GPI-Anchored Metastasis-Associated Protein C4.4A Homolog, LY6/PLAUR Domain Containing 3
- Isotype
- Mouse IgG2b, κ
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IHC staining of anti-human LYPD3 (clone 11A1) Alexa Fluor® 594 on formalin-fixed paraffin-embedded human skin tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human LYPD3 (clone 11A1) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. -
IHC staining of anti-human LYPD3 (clone 11A1) Alexa Fluor® 594 on formalin-fixed paraffin-embedded human brain tissue (negative control). After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human LYPD3 (clone 11A1) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 364503 | 25 µg | $190.00 | |||
| 364504 | 100 µg | $475.00 | |||
C4.4A, also known as Ly6/PLAUR domain containing 3 (LYPD-3), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR). C4.4A is also expressed in the placenta, skin and urothelium and can be found in suprabrasal layers of stratified squamous epithelium and is upregulated on migrating keratinocytes during wound healing. Human C4.4A mRNA has been detected in cancer cell lines, including melanoma, breast, bladder, and renal cell carcinoma, as well as in tumor tissue samples from malignant melanoma, colorectal cancer (CRC), breast cancer, lung carcinoma, and urothelial tumors. Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - RRID
-
AB_3097220 (BioLegend Cat. No. 364503)
AB_3097220 (BioLegend Cat. No. 364504)
Antigen Details
- Distribution
-
Placenta, skin, urothelium, keratinocytes
- Function
- Interacting selectively and non-covalently with laminins, glycoproteins that are major constituents of the basement membrane of cells
- Interaction
- LGALS3 (By similarity). AGR2 and AGR3
- Ligand/Receptor
- Laminin-1 and laminin-5
- Biology Area
- Cell Adhesion
- Molecular Family
- Adhesion Molecules
- Antigen References
-
- Smith BA, et al. 2001. Cancer Res. 61:1678-85.
- Würfle J, et al. 2001. Gene. 262:35-41.
- Gene ID
- 27076 View all products for this Gene ID
- UniProt
- View information about LYPD3 on UniProt.org
Other Formats
View All LYPD3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human LYPD3 | 11A1 | IHC-P,FC |
| Alexa Fluor® 594 anti-human LYPD3 | 11A1 | IHC-P |
| Alexa Fluor® 647 anti-human LYPD3 | 11A1 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human LYPD3
Human paraffin-embedded skin tissue slices were prepared wit... 
MCF-7 cells were stained with purified anti-human LYPD3 (clo... -
Alexa Fluor® 594 anti-human LYPD3
IHC staining of anti-human LYPD3 (clone 11A1) Alexa Fluor® 5... 
IHC staining of anti-human LYPD3 (clone 11A1) Alexa Fluor® 5... -
Alexa Fluor® 647 anti-human LYPD3
IHC staining of Alexa Fluor® 647 anti-human LYPD3 (clone 11A... 
MCF-7 cells were stained with anti-human LYPD3 (clone 11A1) ...