- Clone
- 10D8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α-tubulin, Tubulin, alpha-ubiquitous chain
- Isotype
- Mouse IgM, κ
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HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-human Tubulin-alpha (clone 10D8) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 627910 | 100 µg | $347.00 | |||
Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full length human α-tubulin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
- RRID
-
AB_2563260 (BioLegend Cat. No. 627910)
Antigen Details
- Structure
- Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
- Distribution
-
Ubiquitous
- Function
- Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
- Interaction
- RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
- Modification
- Phosphorylation, acetylation
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96. - Gene ID
- 84790 View all products for this Gene ID
- UniProt
- View information about Tubulin-alpha on UniProt.org
Other Formats
View All Tubulin-α Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Tubulin-α | 10D8 | WB,ICC,IHC-P |
| Alexa Fluor® 488 anti-Tubulin-α | 10D8 | ICC |
| Alexa Fluor® 647 anti-Tubulin-α | 10D8 | ICC |
| Alexa Fluor® 594 anti-Tubulin-α | 10D8 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tubulin-α
Whole cell lysates (15 µg protein) from HeLa (lane 1), Jurka... 
IHC staining using purified anti-alpha tubulin (clone 10D8) ... -
Alexa Fluor® 488 anti-Tubulin-α
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten... -
Alexa Fluor® 647 anti-Tubulin-α
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ... -
Alexa Fluor® 594 anti-Tubulin-α
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ...