Alexa Fluor® 594 anti-Vimentin Antibody

Pricing & Availability
Clone
W16220A (See other available formats)
Regulatory Status
RUO
Other Names
Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113
Isotype
Rat IgG2a, κ
W16220A_A594_Vimentin_Antibody_020618
NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 594 Rat IgG2b (Cat. No. 400661) or with Alexa Fluor® 594 anti-Vimentin antibody (red) at 1: 250 (2 µg/ml, Figure B) and 1: 1000 (0.5 µg/ml, Figure C) dilution. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • W16220A_A594_Vimentin_Antibody_020618
    NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 594 Rat IgG2b (Cat. No. 400661) or with Alexa Fluor® 594 anti-Vimentin antibody (red) at 1: 250 (2 µg/ml, Figure B) and 1: 1000 (0.5 µg/ml, Figure C) dilution. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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699303 25 µg $136.00
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699304 100 µg $347.00
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Description

Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5 and rheumatoid arthritis6, and also found after transplantation7.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody does not cross-react with human (in-house tested).

May be weakly reactive with human Vimentin by WB.

RRID
AB_2728517 (BioLegend Cat. No. 699303)
AB_2728517 (BioLegend Cat. No. 699304)

Antigen Details

Structure
466 amino acids with a predicted molecular weight of 54 kD.
Distribution

Cytoplasm.

Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Interaction
HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1.
2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345.
3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429.
4. Scanlon CS, et al. 2013. J Dent Res. 92:114.
5. Thebault S, et al. 2002. J Immunol. 169:4046.
6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142.
7. Rose ML. 2013. Hum Immunol. 74:1459.

Gene ID
22352 View all products for this Gene ID
UniProt
View information about Vimentin on UniProt.org
Go To Top Version: 1    Revision Date: 02/07/2018

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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