Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG Antibody

Pricing & Availability
Clone
Poly4055 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Poly4055_AF594_GoatAntiHamsterIgG_Antibody_IHCF_082316
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of purified CD3ε (clone 145-2C11) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG (red) and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) for two hours at room temperature. The image was captured with a 10X objective.
  • Poly4055_AF594_GoatAntiHamsterIgG_Antibody_IHCF_082316
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of purified CD3ε (clone 145-2C11) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG (red) and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) for two hours at room temperature. The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 594 spectral data See high resolution IHC-F data...
Cat # Size Price Quantity Check Availability
405512 100 µg $165.00
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Description

This polyclonal antibody reacts with the heavy chains of IgG and the light chains common to most immunoglobulins from Armenian hamster. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with mouse, rat, human, bovine, and rabbit immunoglobulins, but may cross-react with Armenian hamster IgM and Syrian hamster immunoglobulins.

Technical data sheet

Product Details

Verified Reactivity
Armenian Hamster
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This conjugated goat anti-hamster IgG antibody is useful for immunofluorescent staining with flow cytometric analysis or microscopy.

Product Citations
  1. Yue R, et al. 2023. Front Immunol. 1289356:14. PubMed
  2. Liu M, et al. 2019. Front Immunol. 10:477. PubMed
RRID
AB_2629746 (BioLegend Cat. No. 405512)
Go To Top Version: 1    Revision Date: 02/08/2017

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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