- Regulatory Status
- RUO
- Other Names
- Streptavidin-Alexa Fluor® 594, Streptavidin-AF594, SAV
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Mouse embryonic fibroblast NIH/3T3 cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with anti-mouse/human CD44 biotinylated antibody (clone IM7), followed by Streptavidin Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 20X objective. -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) purified, CD3 (clone 17A2) Alexa Fluor® 647 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Biotin for 2 hours, followed by 2.5 µg/ml of Streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405240 | 100 µg | $265.00 | |||
Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 594 is useful for detecting biotinylated antibodies.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Streptavidin was conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The streptavidin-Alexa Fluor® 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICC - Quality tested
IHC-F - Verified
IHC-P - Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 594 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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Streptavidin-Alexa Fluor® 594 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 594 is 60 kD and Streptavidin alone is 52 kD.
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Application References
(PubMed link indicates BioLegend citation) -
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
- Nishimoto KP,et al. 2008. J. Immunol. 181:4010. PubMed
- Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
- Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
- Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
- Yamakawa N, et al. 2012. Int Immunol. PubMed
- Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
- Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
- Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
- Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
- Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
- Reading JL, 2013. J. immunol. 190:4542. Pubmed
- Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
- Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
- Product Citations
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Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org