- Clone
- 7D6-A9-D4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos
- Isotype
- Mouse IgG1, κ
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HeLa cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail (without Brefeldin A)(Cat. No. 423301) for 4 hours (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-c-Fos (clone 7D6-A9-D4) or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 400130). -
HeLa cells untreated (top panels), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) for 4 hours (bottom panels) were fixed and permeabilized with 2% PFA and ice-cold methanol, and then intracellularly stained with Alexa Fluor® 647 anti-c-Fos (clone 7D6-A9-D4)(right panels) or Alexa Fluor® 647 mouse IgG1, κ isotype control (Cat. No. 400130) (left panels). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) and Alexa Fluor® 488 anti-Cytokeratin (clone C-11) (Cat. No. 628608) (green). Scale bar 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 600755 | 25 µg | $195.00 | |||
| 600756 | 100 µg | $475.00 | |||
The c-Fos protein, or FBJ murine viral (v-Fos) oncogene homolog, is a member of the Fos family of transcription factors. C-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the AP-1 (Activator Protein-1) complex, which regulates critical cellular processes, including cell proliferation, differentiation, and survival. C-Fos has been demonstrated to play a critical role in angiogenesis, bone development, and in the progression of cancer. C-Fos is also often used as an indirect marker of neuronal activity.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant human FOS protein fragments
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
For ICC we recommend PFA-MeoH fixation/permeabilization. We do not recommend methanol-only and PFA+ Triton X-100 fixation/permeabilization due to dim signal.
For ICFC, we recommend fixation/permeabilization with the True-Phos™ Perm Buffer. Cytokine method was tried and failed due to no signal.
- RRID
-
AB_3097469 (BioLegend Cat. No. 600755)
AB_3097469 (BioLegend Cat. No. 600756)
Antigen Details
- Structure
- c-Fos is a 330 amino acid protein with a predicted molecular weight of 41 kD.
- Distribution
-
Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes
- Function
- FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.
- Interaction
- Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors
- Antigen References
-
- Dony C and Gruss P. 1987. Nature. 328:711-4.
- Grigoriadis AE, et al. 1994. Science. 266:443-8.
- Glover JN and Harrison SC. 1995. Nature. 373:257-61.
- Gene ID
- 2353 View all products for this Gene ID
- UniProt
- View information about c-Fos on UniProt.org
Other Formats
View All c-Fos Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-c-Fos | 7D6-A9-D4 | ICC,ICFC |
| Alexa Fluor® 488 anti-c-Fos | 7D6-A9-D4 | ICFC |
| Alexa Fluor® 647 anti-c-Fos | 7D6-A9-D4 | ICFC,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-c-Fos
ICC staining of purified anti-c-Fos (clone 7D6-A9-D4) (panel... 
HeLa cells treated (positive control, filled histogram) or u... -
Alexa Fluor® 488 anti-c-Fos
HeLa cells untreated (negative control, open histogram), or ... -
Alexa Fluor® 647 anti-c-Fos
HeLa cells untreated (negative control, open histogram), or ... 
HeLa cells untreated (top panels), or treated with Cell Acti...