Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (for Flow Cytometry) Antibody

Pricing & Availability
Clone
A22009C (See other available formats)
Regulatory Status
RUO
Other Names
G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1
Isotype
Mouse IgG2b, κ
A22009C_A-647_c-Fos_ICFC_070524
HeLa cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (clone A22009C) or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 400330).
  • A22009C_A-647_c-Fos_ICFC_070524
    HeLa cells untreated (negative control, open histogram), or treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423301) (positive control, filled histogram) were fixed and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (clone A22009C) or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 400330).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
631257 25 tests $175.00
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631258 100 tests $430.00
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Description

The proto-oncogene c-Fos is the human homolog of the retroviral v-Fos oncogene, and is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the Activator Protein-1 complex (AP-1). This complex is involved in critical cellular processes such as inflammation, cell proliferation, differentiation, survival, hypoxia, bone development and angiogenesis which suggests c-Fos is an important therapeutic target for cancer, vascular remodeling, acute inflammation and autoimmune diseases.  In addition, increases in c-Fos expression and activation are associated with neuronal activity induced by psychoactive drugs. Expression of c-Fos is rapid and transitory, and phosphorylation at residues Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human c-Fos phosphorylated at Ser32.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For ICFC use we recommend cell fixation/permeabilization with True-Phos™ Perm buffer.

We recommend this antibody for use in intracellular staining for flow cytometry (ICFC) but not for use in immunocytochemistry (ICC) and immunohistochemistry (IHC).

For use in ICC and IHC on FFPE tissues (IHC-P), we recommend Cat. No. 631259 and 631260. This antibody is the same clone but developed with optimal F/P ratio for imaging.

RRID
AB_3662313 (BioLegend Cat. No. 631257)
AB_3662313 (BioLegend Cat. No. 631258)

Antigen Details

Structure
c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins.
Distribution

Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation/phosphorylation the protein is translocated to the nucleus.

Function
c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus.
Interaction
c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor.
Ligand/Receptor
As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription
Biology Area
Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors
Molecular Family
Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors
Antigen References
  1. Sasaki T, et al. 2006. Mol Cell. 24:63-75.
  2. Huang K, et al. 2014. Cell Signal. 26:629-38.
  3. Qu X, et al. 2019. Cancer Sci. 110:3183-3196.
  4. Guo JC, et al. 2015. PLoS One. 10:e0120332.
  5. Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818.
Gene ID
2353 View all products for this Gene ID
UniProt
View information about c-Fos on UniProt.org
Go To Top Version: 1    Revision Date: 07/26/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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